FastQCFastQC Report
Fri 14 Jul 2023
SRR9691582_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691582_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12771989
Total Bases970.6 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA1688321.3218927764500894No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC1058290.8286023422037084No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA1025990.8033126242122508No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA952490.7457648139220915No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG906370.7096545416692732No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG837750.6559275927970186No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC737650.5775529559256589Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG660350.5170298846953282No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC649770.5087461318671666No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT532200.416693124305071Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA474910.3718371508149592Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA470680.36852521561050516Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA461840.3616038191075799Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG408070.3195038768041532No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA405890.3177970165805811Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCTGATCTCGTATG402810.3153854892922316TruSeq Adapter, Index 9 (97% over 49bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG354030.2771925343812933No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA341770.2675934030322137No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA340700.2667556321885338No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA317200.24835599216378904No Hit
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG316540.2478392363162856No Hit
AGTTGTGTGGACCGCAAAACAGCATTTTAGCAGATCGGAAGAGCACACGT293710.22996418177309735No Hit
AGCGTTAATGGTCGTAGCGTTCTGACCGCATTTGATCTGAGATCGGAAGA291760.2284374031327462No Hit
AGCTCTTGATCCGGCAAACAAACCACCGCTAGATCGGAAGAGCACACGTC262930.20586456815770826No Hit
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA255570.20010195749463922No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC253390.1983950972710672No Hit
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC251440.196868318630716No Hit
TTGTGTGGACCGCAAAACAGCATTTTAGCAGATCGGAAGAGCACACGTCT241070.18874898811766908Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA236880.18546837144942735No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT233430.18276714770111374No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGAGATCGGAAGA230350.1803556204127642No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG213460.16713136849710722No Hit
ATCTGCTGGAACGTATTTATCATACCTGTAAACCGGGAGATCGGAAGAGC207320.16232397318851433No Hit
AACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAG201850.158041163361478No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC199270.15602111777578262Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA194450.15224723416219665No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG193060.15115891502881815No Hit
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA188280.14741634995144454TruSeq Adapter, Index 9 (100% over 35bp)
GGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCAC177510.13898383407627427No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT172250.13486544656435268No Hit
ACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGGA165470.12955695467636247No Hit
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA165310.12943168053151313Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC165260.12939253236124773No Hit
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA165090.12925942858234532Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCGTTAATGGTCGTAGCGTTCTGACCGCATTTGATCAGATCGGAAGAGC160260.12547771533470628No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT160050.12531329301959154Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCACTTCACTCTGAAGTCTGACGTTCTGAGATCGGAAGAGCACACGTCT158300.12394310706030204Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGAGATCGGAAGAGCA153490.12017705308076916No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG152990.11978557137811503Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA146280.1145318869284964Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGG145450.11388202730209054No Hit
AGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCA143230.11214384854230613No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC141710.11095374416623753Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG138540.10847175017141025No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA137700.1078140609109513No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA134290.10514415569885004No Hit
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG133530.10454910351081574No Hit
ATCTGCTGGAACGTATTTATCATACCTGTAAACCGGGTAGATCGGAAGAG131140.10267782097212892No Hit
ACTCGGCTGCGAACCGAGCGGTCGGAGGTTCGAGATCGGAAGAGCACACG131130.10266999133807585No Hit
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGAGATCGGA129600.10147205732795417No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA129430.10133895354905176TruSeq Adapter, Index 9 (100% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)