FastQCFastQC Report
Fri 14 Jul 2023
SRR9691585_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691585_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4277223
Total Bases325 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC341600.7986490299897854No Hit
ATCTGCTGGAACGTATTTATCATACCTGTAAACCGGGAGATCGGAAGAGC327160.764888807527688No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA283760.6634211028978382No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG276410.6462370561460088No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA235660.5509649602089954No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC218680.5112663052639528No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG202190.47271325343569887No Hit
AGCGTTAATGGTCGTAGCGTTCTGACCGCATTTGATCAGATCGGAAGAGC192710.45054934007415554No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA154780.361870306972538No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC151820.3549499289609169Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG151250.353617288600571No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG147500.3448499178088213No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA145330.33977653257732876Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA144610.33809319738531285No Hit
ATCTGCTGGAACGTATTTATCATACCTGTAAACCGGGTAGATCGGAAGAG141170.3300505959123478No Hit
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG108940.2546979664141898No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA106580.24918036772924862Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCGTTAATGGTCGTAGCGTTCTGACCGCATTTGATCTGAGATCGGAAGA102450.23952457003060162No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG90990.21273148489101457No Hit
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA89140.2084062486337514No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA87530.2046421241071602No Hit
ATCTGCTGGAACGTATTTATCATACCTGAGATCGGAAGAGCACACGTCTG86130.20136897234490697Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC85530.199966193018227No Hit
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA83820.19596827193718913No Hit
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC81130.18967914462257404No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA76880.17974279105859106Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC74860.17502010065876855Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT73750.17242495890441065Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA71960.16824000057981547No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG68940.16117934463552638No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAAGTATCTCGTATG64650.1511494724497647TruSeq Adapter, Index 3 (97% over 36bp)
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT60970.1425457592461277Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCATCTGCTGGAACGTATTTATCATACCTGTAAACCGGGAGATCGGAA58580.13695802159485257No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA57610.13469019501671997Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGTTAGTGGACGCACACTTATAGACAAGATCGGAAGAGCACACGTCTGA57530.13450315777316263Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA56020.1309728298010181No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA55770.13038833841490144No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA55280.1292427352981128No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG54850.1282374101139922No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC51850.12122351348059243No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG50150.11724897205499923No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC49540.11582281307287462Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC48890.11430313546897135No Hit
ATCTGCTGGAACGTATTTATCATACCTGTAGATCGGAAGAGCACACGTCT47680.11147419716016677Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGAGATCGGA47650.11140405819383278No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAGATCGGAAGAGCAC47210.11037535335426747No Hit
ATCTGCTGGAACGTATTTATCATACCTGTAAACCGGGTGGAGATCGGAAG45410.10616701537422762No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC44280.10352511430898037No Hit
AGCCTGTATCCGGGTTGTAAAATTACCGTTTTTGATAAGATCGGAAGAGC43710.10219247394863444No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA43480.1016547418734071No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA43030.10060265737839715Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC43020.10057927772295248Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)