FastQCFastQC Report
Fri 14 Jul 2023
SRR9691587_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691587_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4568583
Total Bases347.2 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC558661.2228299234138902Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA475421.0406290090384698Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC443030.969731752711946No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA387210.847549448045488No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC297900.6520621383041525No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG275830.6037539429621832No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG263380.5765026048558163No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT219290.479995657296803Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA207240.4536198641898374No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG201840.44180000669791925No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG168210.3681885608732511No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA166190.3637670586262743Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA161030.3524725281339969No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA157590.34494284113914536Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA129980.28450834755546744No Hit
AAGTTAGTGGACGCACACTTATAGACAAGATCGGAAGAGCACACGTCTGA123900.271200063564567Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA112170.2455247064571225Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACATATCTCGTATG111970.24508693395742182TruSeq Adapter, Index 4 (97% over 37bp)
ACTTCGAGGACATCACTATGACTGAAGATCGGAAGAGCACACGTCTGAAC110200.24121264733507086Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA106240.23254475184099752No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA100130.219170801975142Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT98590.21579995372744679No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC94710.20730716723325374Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC87910.19242290224343084Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA85080.18622842137266632No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA82700.1810189286262283No Hit
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT79580.1741896776308978Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC79180.17331413263149645No Hit
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA75510.16528100726198913No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG73910.16177882726438372No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC73210.16024662351543137Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG70700.15475257864418793No Hit
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT67730.1482516570236329Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT65560.14350182540188064Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA63520.13903654590493375No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT63340.13864255065520317No Hit
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC62480.13676012890649025Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA62370.13651935403165488No Hit
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG61410.13441804603309165TruSeq Adapter, Index 4 (100% over 35bp)
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC61410.13441804603309165No Hit
TAGTCACTTCGAGGACATCACTATGACTGATTTCTTAGAGATCGGAAGAG61030.13358627828366038No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC60470.1323605152844985Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC56890.12452438753985645No Hit
TGTGGGCACTCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT56200.12301407241588913Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGAGATCGGAAGA55020.12043121466765515No Hit
ACTCGGCTGCGAACCGAGCGGTCGGAGGTTCGAGATCGGAAGAGCACACG53610.11734491854476542No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG52270.1144118427967709Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGTTAGTGGACGCACACTTATAGACAAGATCGGAAGAGCACACGTCTGAA50480.11049377892444988Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT48630.10644438330221866Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA47780.1045838501784908No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC47450.10386152555398467No Hit
ACTTCGAGGACATCACTATGACTGATAGATCGGAAGAGCACACGTCTGAA47100.1030954236795085Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGGA46890.10263576255482279No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC46130.10097222705596023No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)