FastQCFastQC Report
Fri 14 Jul 2023
SRR9691591_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691591_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4024160
Total Bases305.8 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA309520.769154307979802No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC298380.7414715120671146No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA285950.7105830782076259No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG249940.6210985646693968No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA249860.6208997654168821Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC226880.5637946801320027No Hit
ATCTGCTGGAACGTATTTATCATACCTGTAAACCGGGAGATCGGAAGAGC181810.4517961512464713No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG171850.42704564430837744No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG169020.42001312075066594No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC158050.3927527732495726Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG132420.32906246272514017No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA126970.3155192636475687No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA125570.3120402767285595Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG118850.29534113951731544No Hit
AAGTTAGTGGACGCACACTTATAGACAAGATCGGAAGAGCACACGTCTGA110230.2739205200588446Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGTTAATGGTCGTAGCGTTCTGACCGCATTTGATCAGATCGGAAGAGC104820.2604767206075305No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT98180.24397638264880123Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACGGATCTCGTATG95680.2377639060077134TruSeq Adapter, Index 4 (97% over 37bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG94720.2353783149775357No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG92490.22983678581368536No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA90560.22504075384676553No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA87870.21835612898095502Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG83860.20839131644865014No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA83310.20702457158761084No Hit
ATCTGCTGGAACGTATTTATCATACCTGTAAACCGGGTAGATCGGAAGAG77300.19208977774243569No Hit
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC76460.1900023855910302No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA74250.18451055624030854No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG72930.18123036857381417No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCACAC72570.18033577193749753No Hit
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA69850.17357659735199396No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC68630.1705449087511431No Hit
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA68120.16927756351636117No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT62120.1543676195777504Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC61110.1518577790147509Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC60270.1497703868633454Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA59910.14887579022702874Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA57780.1435827601288219No Hit
ACTTCGAGGACATCACTATGACTGAAGATCGGAAGAGCACACGTCTGAAC54950.13655023657111048Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCTGCTGGAACGTATTTATCATACCTGAGATCGGAAGAGCACACGTCTG54260.13483559301817025Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGTTAATGGTCGTAGCGTTCTGACCGCATTTGATCTGAGATCGGAAGA53980.13413979563436843No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT53430.1327730507733291No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA51450.12785276927358755No Hit
AGTGCCTGTAGTCACTTCGAGGACATCACAGATCGGAAGAGCACACGTCT49770.1236779849707765Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA49730.12357858534451911Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG47110.11706790982465906No Hit
GATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCA45880.11401137131724386No Hit
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC45660.11346467337282813Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAA45100.11207307860522445No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCA44770.11125303168860086No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGAGATCGGAAGAGCA44350.11020933561289808No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA43820.10889229056498748No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC40940.1017355174744543Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGGA40710.10116396962347421No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG40330.10021967317402887TruSeq Adapter, Index 11 (100% over 35bp)

[FAIL]Adapter Content

Adapter graph