FastQCFastQC Report
Fri 14 Jul 2023
SRR9691592_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691592_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3934336
Total Bases299 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA296400.753367277222891No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC288810.7340755848000781No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA284940.7242391092169047No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA247360.6287210853368904Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG239580.6089464651722679No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC222500.5655338029085467No Hit
ATCTGCTGGAACGTATTTATCATACCTGTAAACCGGGAGATCGGAAGAGC177110.4501649071152032No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG168930.4293735969678238No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG166510.42322262257214427No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC157910.40136378794287014Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG127990.3253153772326512No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA127120.3231040765201548No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA123470.3138267804274978Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG116780.296822640465888No Hit
AAGTTAGTGGACGCACACTTATAGACAAGATCGGAAGAGCACACGTCTGA108400.27552298532713015Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGTTAATGGTCGTAGCGTTCTGACCGCATTTGATCAGATCGGAAGAGC103610.26334812278361586No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT96000.2440055958616651Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACGGATCTCGTATG91860.2334828545401308TruSeq Adapter, Index 4 (97% over 37bp)
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG91500.23256783355564956No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG90520.23007694309789503No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA87950.22354470995868173Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA87090.2213588264957543No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG83350.21185277515697692No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA80920.20567638351172854No Hit
ATCTGCTGGAACGTATTTATCATACCTGTAAACCGGGTAGATCGGAAGAG75340.19149355825226927No Hit
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC74500.1893585092884797No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA72960.18544425285486546No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG72270.18369046263460975No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCACAC68850.17499776328203795No Hit
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA68830.17494692878290008No Hit
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA68820.17492151153333116No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC67050.17042265835963172No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT62780.15956949279370142Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA59740.15184264892474866Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC59740.15184264892474866Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC58740.14930092396785632Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA57690.14663211276311935No Hit
ACTTCGAGGACATCACTATGACTGAAGATCGGAAGAGCACACGTCTGAAC54540.13862567914890847Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCGTTAATGGTCGTAGCGTTCTGACCGCATTTGATCTGAGATCGGAAGA53400.1357281126980512No Hit
ATCTGCTGGAACGTATTTATCATACCTGAGATCGGAAGAGCACACGTCTG51720.13145801477047206Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA50850.12924671405797572Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT50450.12823002407521877No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA49860.1267304063506523No Hit
AGTGCCTGTAGTCACTTCGAGGACATCACAGATCGGAAGAGCACACGTCT48000.12200279793083255Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC45340.11524180954549891Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAA44620.11341176757653643No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGAGATCGGAAGAGCA44280.11254758109119302No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCA44140.11219173959722811No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG43820.11137838761102255No Hit
GATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCA43380.11026002862998992No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA42440.1078708071705111No Hit
ATGAAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACA40520.10299069525327781No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA40240.10227901226534795No Hit
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA39780.10110981878517747Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGGA39400.10014396330155838No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)