FastQCFastQC Report
Fri 14 Jul 2023
SRR9691594_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691594_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3983381
Total Bases302.7 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA302360.7590536782697914No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC291760.7324431180446962No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA282470.7091212213945892No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG247370.6210051210265852No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA243340.6108880872806293Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC221040.5549054935995327No Hit
ATCTGCTGGAACGTATTTATCATACCTGTAAACCGGGAGATCGGAAGAGC178250.44748418491728514No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG170040.4268735528938859No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG165190.41469796637579986No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC154460.3877610502234158Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG131290.32959438225969345No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA126890.31854848933606905No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA122240.30687498885996595Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG117760.29562826151954835No Hit
AAGTTAGTGGACGCACACTTATAGACAAGATCGGAAGAGCACACGTCTGA108080.27132729708757464Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGTTAATGGTCGTAGCGTTCTGACCGCATTTGATCAGATCGGAAGAGC101610.25508481362942687No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT97980.24597195196743668Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG94680.23768753227471837No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACGGATCTCGTATG94530.2373109677432312TruSeq Adapter, Index 4 (97% over 37bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA91060.22859977491482739No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG89700.22518558982934345No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA87630.21998899929482016Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG83360.20926946229848464No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA78000.19581355637334213No Hit
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC75500.18953748084855554No Hit
ATCTGCTGGAACGTATTTATCATACCTGTAAACCGGGTAGATCGGAAGAG74590.1872529893575332No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA73030.18333671823006636No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCACAC71170.17866731803962513No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG70090.1759560534129173No Hit
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA69230.17379708343239073No Hit
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA68950.17309416297361463No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC66690.16742059069920753No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT63200.1586591892666054Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC60940.15298561699219834Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC60710.15240821804391796Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA58880.14781413075977418Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA56620.14214055848536708No Hit
ACTTCGAGGACATCACTATGACTGAAGATCGGAAGAGCACACGTCTGAAC53410.13408207751154108Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCGTTAATGGTCGTAGCGTTCTGACCGCATTTGATCTGAGATCGGAAGA53310.13383103449054962No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT52790.132525610781394No Hit
ATCTGCTGGAACGTATTTATCATACCTGAGATCGGAAGAGCACACGTCTG51920.13034153649876826Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA50850.1276553761741596No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA50220.12607380514191335Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGCCTGTAGTCACTTCGAGGACATCACAGATCGGAAGAGCACACGTCT48630.12208222110814909Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG46660.11713667359461724No Hit
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC45680.11467645198890088Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCA44910.11274342072726662No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGAGATCGGAAGAGCA44630.11204050026849051No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA44400.11146310132021013No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCA44320.11126226690341698No Hit
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAA42630.10701963984866122No Hit
ACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGGA40970.10285232570020292No Hit
ATGAAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACA40090.10064314711547802No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)