FastQCFastQC Report
Fri 14 Jul 2023
SRR9691597_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691597_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2217952
Total Bases168.5 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA585782.6410851091457346No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG341141.5380855852606368No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC339381.5301503368873628No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG326401.4716278801344664No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA279511.2602166322805903Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA155810.702494914227179No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC149520.6741354186204209No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA119350.5381090303126488Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA114660.5169633968634127Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG102810.46353573025926625No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG86880.39171271515343886No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA82500.3719647674972226No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA82230.37074742825814083No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC77930.35136017370980077Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT75010.3381948752723233No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA74680.3367070162023344No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGACTATC73170.32989893379117313TruSeq Adapter, Index 19 (97% over 37bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA70580.3182214944236845Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC68080.30694983480255655Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA66700.30072787869169393No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT66580.3001868390298798No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA61730.2783198193648916No Hit
AAACTGCGACGACACATTCACAGATCGGAAGAGCACACGTCTGAACTCCA43300.19522514463793628Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC41880.1888228419731356Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGACTAT37630.16966102061721805TruSeq Adapter, Index 19 (97% over 37bp)
TGTGGGCACTCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTG35000.15780323469579144TruSeq Adapter, Index 10 (97% over 36bp)
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG34160.15401595706309243No Hit
TGCGTCTGGATCGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC33190.14964255313009478Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAACTGCGACGACACATTCACCAGATCGGAAGAGCACACGTCTGAACTCC32510.14657666171314798Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG32310.14567492894345774No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC31470.14188765131075876No Hit
AAACTGCGACGACACATTCAGATCGGAAGAGCACACGTCTGAACTCCAGT31360.14139169828742912Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAG29770.13422292276839173No Hit
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC28380.1279558800190446Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAAACTGCGACGACACATTCACAGATCGGAAGAGCACACGTCTGAACTCC27860.12561137481784998Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG27670.12475472868664426No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC27460.1238079092784695Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT27290.12304143642423282Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA27240.12281600323181024No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA26710.12042641139213112Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCAGATCGGAAGAGCACACGT26660.12020097819970856No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC26170.11799173291396749No Hit
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT25690.1158275742667109TruSeq Adapter, Index 3 (100% over 35bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG25220.11370850225793885No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGACTATCTCGTATG24200.10910966513251864TruSeq Adapter, Index 19 (97% over 37bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT23770.10717093967768462Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC23530.10608886035405635No Hit
ACCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAGATCGGAAGA22980.1036090952374082No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGA22970.1035640085989237Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)