FastQCFastQC Report
Fri 14 Jul 2023
SRR9691599_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691599_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2882914
Total Bases219.1 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA781742.7116313563290477No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA452661.5701474272212075Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG421701.4627560863764928No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC314761.0918119652546001No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC313831.088586062574187No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG255140.8850073224522134No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA173330.6012319479526618Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA167870.5822927773773342Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA158130.5485075170469879No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA138360.47993106974401595No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG136930.4749708107838111No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA122100.4235298035251832No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA119500.4145111508702653No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT115300.39994255812001334No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA102140.35429430083589036No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA94890.32914613477890775Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC87980.3051773309921836Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC76870.26663993445520745Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG75870.2631712218956237No Hit
TGCGTCTGGATCGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC72590.2517938447001888Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC71930.24950449441086348No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC71520.24808232226143412Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG64780.22470319960983923TruSeq Adapter, Index 11 (100% over 35bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT62850.21800858436984244No Hit
AAACTGCGACGACACATTCACAGATCGGAAGAGCACACGTCTGAACTCCA62340.21623954096445472Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTGGGCACTCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGA61450.21315238678642512TruSeq Adapter, Index 2 (97% over 36bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA51670.17922837795369548No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG51560.17884681957214124No Hit
AAACTGCGACGACACATTCACCAGATCGGAAGAGCACACGTCTGAACTCC51480.17856932256737454Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC46620.16171137952779724No Hit
AAAACTGCGACGACACATTCACAGATCGGAAGAGCACACGTCTGAACTCC46200.160254520252772Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAA45260.15699393044676324No Hit
AAACTGCGACGACACATTCAGATCGGAAGAGCACACGTCTGAACTCCAGT43490.15085430921629991Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA42700.14811402629422868No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG42250.14655310564241597No Hit
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGA42120.14610217300967007No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG40420.1402053616583776Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG39840.138193508373819No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC39800.13805475987143562Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA39700.13770788861547725Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT38800.13458604731185184No Hit
AACGTAGCGGTTTTACCGTTCGTCCGGTTGCAGGTTATCTGAGATCGGAA38770.1344819859350643No Hit
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC37570.13031953086356374Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTGGGCACTCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG36780.12757924794149253Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCAGATCGGAAGAGCACACGT36070.12511646202418802No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAACTATCTCGTATG36030.12497771352180467TruSeq Adapter, Index 2 (97% over 36bp)
ACACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG35550.12331273149320446Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC34750.1205377614455374No Hit
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT34480.11960120905444976Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGT34010.11797091415144537No Hit
ATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGAGATCGGA33860.1174506072675078No Hit
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA33650.1167221776299952Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA33170.11505719560139499No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG32880.11405126895911567No Hit
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGAGATCGGA32400.11238628693051543No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGA32360.1122475384281321Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAACTGCGACGACACATTCACCAGATCGGAAGAGCACACGTCTGAACTC32300.11203941567455705Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGGCATCTGTATGAACATACCGATAATCTGTGAGATCGGAAGAGCACA31500.10926444562689001No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG31130.10798102197984401No Hit
TCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACA30540.10593448156968954No Hit
AAAACTGCGACGACACATTCAGATCGGAAGAGCACACGTCTGAACTCCAG30150.10458168367145186Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCA30030.10416543816430181No Hit
TGTGGGCACTCGAAGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC29830.10347169565238505Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGAA29620.10274326601487245Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG29210.1013210938654431No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)