FastQCFastQC Report
Fri 14 Jul 2023
SRR9691600_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691600_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2802729
Total Bases213 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA760242.7124991392317988No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA433401.546350003871227Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG402831.4372777389465767No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC307361.0966454480615144No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC303881.0842289782565493No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG246480.8794285854964929No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA168220.6002007329285136Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA161750.57711608935434Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA151070.5390103716770334No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA133310.4756435602585908No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG131830.4703629926403873No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA117960.420875510975196No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA113920.40646098855793766No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT110570.39450835239511206No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA99560.35522521085698977No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA92690.3307133868454638Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC85150.3038110356013728Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC73810.26335047020243485Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC69430.24772284441342707No Hit
TGCGTCTGGATCGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC68990.24615294593233952Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC65730.23452142536791817Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG62590.22331805893470255TruSeq Adapter, Index 11 (100% over 35bp)
AAACTGCGACGACACATTCACAGATCGGAAGAGCACACGTCTGAACTCCA61230.21846564544770472Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61090.2179661322946314No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT60790.21689574696661718No Hit
TGTGGGCACTCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGA59420.21200765396868554TruSeq Adapter, Index 2 (97% over 36bp)
AAACTGCGACGACACATTCACCAGATCGGAAGAGCACACGTCTGAACTCC51320.18310725011230128Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA50450.18000313266105997No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG49110.17522207819592975No Hit
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC44990.16052211969120098No Hit
AAAACTGCGACGACACATTCACAGATCGGAAGAGCACACGTCTGAACTCC43720.15599082180260737Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACTGCGACGACACATTCAGATCGGAAGAGCACACGTCTGAACTCCAGT42940.15320781994977037Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAA42200.15056753614066862No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG41210.14703526455822163No Hit
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG40160.14328891591017182No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA39890.14232556911495903Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG39610.1413265428088124Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA39600.1412908632978786No Hit
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGA39380.1405059140573348No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC39000.13915009264185013Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT37400.1334413708924409No Hit
AACGTAGCGGTTTTACCGTTCGTCCGGTTGCAGGTTATCTGAGATCGGAA36990.13197851094415478No Hit
ACACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG36310.12955230420065586Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCAGATCGGAAGAGCACACGT36020.12851759838357543No Hit
TGTGGGCACTCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG35540.12680498185875266Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC34810.12420037756058472Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT34290.1223450429920267Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAACTATCTCGTATG33720.12031131086879965TruSeq Adapter, Index 2 (97% over 36bp)
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA33200.11845597630024167Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGAGATCGGA32090.114495550586589No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC32000.11417443498818473No Hit
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGAGATCGGA31310.111712548733752No Hit
AAAACTGCGACGACACATTCACCAGATCGGAAGAGCACACGTCTGAACTC31210.11135575362441393Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG30760.10975017563239256No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG30670.10942906003398829No Hit
AAAACTGCGACGACACATTCAGATCGGAAGAGCACACGTCTGAACTCCAG30530.108929546880915Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGT30360.10832299519504027No Hit
TCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACA30280.1080375591075698No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA30150.10757372546543031No Hit
ATCGGCATCTGTATGAACATACCGATAATCTGTGAGATCGGAAGAGCACA29980.10696717377955557No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGA29710.10600382698434277Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGGGCACTCGAAGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC29510.10529023676566661Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCGTGTATGGACTGACGAAGAATCAGATCGGAAGAGCACACGTCTGAAC28640.10218611931442534Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGAA28590.1020077217597563Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCA28120.10033078474586733No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)