FastQCFastQC Report
Fri 14 Jul 2023
SRR9691604_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691604_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1776407
Total Bases135 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA174980.9850220135363124No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG171230.9639119863860026No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA149940.8440633255779784Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC131030.737612495334684No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC118860.6691034205562126No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA98990.5572484233624388Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG82810.4661656928845698No Hit
TTCTGCGTCGCGAAACTCTGGAAGATGCAGATCGGAAGAGCACACGTCTG73620.4144320530148778Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA71670.4034548388967168Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA69980.3939412533276439No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA61270.34490969693319157No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA53570.3015637745178892No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCTGATC47960.26998317390102605TruSeq Adapter, Index 9 (97% over 42bp)
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT41770.2351375557515817Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGGCATCTGTATGAACATACCGATAATCTGTGAGATCGGAAGAGCACA34930.19663286622941703No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG34490.19415595637711403No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCACAC33890.1907783520330645No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG33530.1887517894266348No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA31880.17946337748049854Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCATGATCGGCATCTGTATGAACATACCGATAATCTGTGAGATCGGAAG31100.17507249183323415No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG29530.16623442713297124No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT28600.16099914039969446No Hit
ATTTCAATCCGTATACCCAGAGCATCGAGATCGGAAGAGCACACGTCTGA28510.160492499748087Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC28110.15824076351872066Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAGATCGGAAGA27950.15734006902697412No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG27940.15728377562123994No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT25910.14585621425720569No Hit
TGATCGGCATCTGTATGAACATACCGATAATCTGTGAGATCGGAAGAGCA25330.14259119672462447No Hit
TTCTGCGTCGCGAAACTCTGGAAGATGAGATCGGAAGAGCACACGTCTGA24400.13735590999134772Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAACGTAGCGGTTTTACCGTTCGTCCGGTTGCAGGTTATCTGAGATCGG24330.1369618561512086No Hit
ATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAGATCGGAA24060.1354419341963863No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG22960.1292496595656288No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT21850.12300109152913719No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCTGATCTCGTATG21800.12271962450046639TruSeq Adapter, Index 9 (97% over 49bp)
ATATCTTCAGGAGATCGTCATGGACAGATTCCCACGAGATCGGAAGAGCA20570.11579553559516484No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGATCGGAAGAGCA19730.11106688951349551No Hit
ATCATGATCGGCATCTGTATGAACATACCGATAATCTGTGTCAGATCGGA19280.10853368625545834No Hit
AGCTTCGGCAGCTTCGGTCTGAAAGCTGTTGGCCGTGGTCGTCTGACTGC19090.10746411154650932No Hit
ATGTGCAGAAACTGGCAACCTGTTATTTCTAGATCGGAAGAGCACACGTC18830.1060004829974212No Hit
ATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGAGATCGGA18570.10453685444833306No Hit
AGGACTGGATGGATTCCAGTAACCCCGGAACCTGGACGGATGAGATCGGA18240.10267917205910582No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)