FastQCFastQC Report
Fri 14 Jul 2023
SRR9691606_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691606_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1705886
Total Bases129.6 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG167880.9841220339459963No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA165640.9709910275364239No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA146990.8616636750638671Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC128230.7516914963836974No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC118190.6928364497979349No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA95870.5619953502168376Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG79620.4667369331831084No Hit
TTCTGCGTCGCGAAACTCTGGAAGATGCAGATCGGAAGAGCACACGTCTG73600.4314473534573823Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA70370.41251291117929334No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA68570.4019612096001726Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA60020.351840627099349No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG52710.308988994575253No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA50220.29439247405746927No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCTGATC47130.27627871967997863TruSeq Adapter, Index 9 (97% over 42bp)
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT40860.2395236258460413Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGGCATCTGTATGAACATACCGATAATCTGTGAGATCGGAAGAGCACA34750.20370646104135914No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCACAC33140.19426855018447892No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG32070.18799614980133492No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA32040.18782028810834958Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG31290.1834237457837159No Hit
ATCATGATCGGCATCTGTATGAACATACCGATAATCTGTGAGATCGGAAG30290.17756168935087105No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG28300.16589619704950975No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT28300.16589619704950975No Hit
ACCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAGATCGGAAGA27760.16273068657577353No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC26390.15469966926277606Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG25260.14807554549366136No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT24650.14449969106962599No Hit
TGATCGGCATCTGTATGAACATACCGATAATCTGTGAGATCGGAAGAGCA24530.14379624429768462No Hit
TTCTGCGTCGCGAAACTCTGGAAGATGAGATCGGAAGAGCACACGTCTGA23500.13775832617185438Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAGATCGGAA23320.1367031560139423No Hit
AGAACGTAGCGGTTTTACCGTTCGTCCGGTTGCAGGTTATCTGAGATCGG22820.1337721277975199No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT21190.12421697581198275No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCTGATCTCGTATG20790.1218721532388448TruSeq Adapter, Index 9 (97% over 49bp)
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG20690.12128594759556033No Hit
ATATCTTCAGGAGATCGTCATGGACAGATTCCCACGAGATCGGAAGAGCA19860.11642044075629908No Hit
ATGTGCAGAAACTGGCAACCTGTTATTTCTAGATCGGAAGAGCACACGTC18350.10756873554270331No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGATCGGAAGAGCA18130.10627908312747746No Hit
AGCTTCGGCAGCTTCGGTCTGAAAGCTGTTGGCCGTGGTCGTCTGACTGC17850.10463770732628089No Hit
ATCATGATCGGCATCTGTATGAACATACCGATAATCTGTGTCAGATCGGA17800.10434460450463864No Hit
ATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGAGATCGGA17740.10399288111866796No Hit
AGGACTGGATGGATTCCAGTAACCCCGGAACCTGGACGGATGAGATCGGA17300.10141357628821622No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)