FastQCFastQC Report
Fri 14 Jul 2023
SRR9691609_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691609_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2032530
Total Bases154.4 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA359101.7667635902053107No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC340681.676137621584921No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA202700.9972792529507559Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA176640.8690646632522029No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG152790.7517232217974642No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC138040.67915356722902No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA137030.6741843908822994Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA127380.6267066168765036No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG115660.5690444913482212No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG100970.49677003537463166No Hit
TTCTGCGTCGCGAAACTCTGGAAGATGCAGATCGGAAGAGCACACGTCTG94430.4645933885354706Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA85300.41967400235174884Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT76620.3769686056294372No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG72390.35615710469218165No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAAGTATC68850.3387403875957551TruSeq Adapter, Index 3 (97% over 36bp)
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG67240.3308192253004876No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG63490.3123693131220695No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG54540.26833552272291183No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT53650.2639567435659006No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA47840.23537167963080496No Hit
AGAACGTAGCGGTTTTACCGTTCGTCCGGTTGCAGGTTATCTGAGATCGG44810.2204641505906432No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG43880.2158885723703955No Hit
TTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAA39250.19310908080077538No Hit
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA39090.19232188454782953No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA35410.17421637073007532No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA33830.1664428077322352Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC33350.1640812189733977No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC32230.15857084520277684Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA31790.1564060555071758No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT30830.15168287798950078Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC30660.15084648197074582TruSeq Adapter, Index 5 (97% over 35bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCACAC29130.14331891780195127No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT28780.14159692599863224Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGTAGCGGTTTTACCGTTCGTCCGGTTGCAGGTTATCTGAGATCGGAA27940.1374641456706666No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA27920.13736574613904837No Hit
ATCATGATCGGCATCTGTATGAACATACCGATAATCTGTGAGATCGGAAG27900.13726734660743015No Hit
ATCGGCATCTGTATGAACATACCGATAATCTGTGAGATCGGAAGAGCACA27670.1361357519938205No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC26880.13224897049490045No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAAGA25740.12664019719266134No Hit
ATGTGCAGAAACTGGCAACCTGTTATTTCTAGATCGGAAGAGCACACGTC24610.1210806236562314No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT23870.1174398409863569No Hit
TTCTGCGTCGCGAAACTCTGGAAGATGAGATCGGAAGAGCACACGTCTGA23780.11699704309407488Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCCATCCTGATCAAATTCAACCAGATCGGTTCTCTGACCGAAACTCTG23330.1147830536326647No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAAGTATCTCGTATG22720.11178186791830871TruSeq Adapter, Index 3 (97% over 36bp)
TGATCGGCATCTGTATGAACATACCGATAATCTGTGAGATCGGAAGAGCA22580.1110930711969811No Hit
ACGTAGCGGTTTTACCGTTCGTCCGGTTGCAGGTTATCTGAGATCGGAAG22070.10858388314071625No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA21860.10755068805872485No Hit
TCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGAA21850.10750148829291573Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTAGATCGGAAGAGCACAC21550.10602549531864229No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC21350.10504150000246Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC20760.10213871381972223No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAGATCGGA20610.10140071733258549No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA20390.10031832248478496Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)