FastQCFastQC Report
Fri 14 Jul 2023
SRR9691610_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691610_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1969857
Total Bases149.7 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA346631.7596708796628384No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC335121.7012402423120052No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA200691.0188049183265588Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA173510.8808253594042614No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG149050.7566539093954536No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA135510.6879179554658029Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC133840.6794401827137706No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA120250.6104504032526219No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG114460.5810574067051568No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG99720.5062296400195547No Hit
TTCTGCGTCGCGAAACTCTGGAAGATGCAGATCGGAAGAGCACACGTCTG92530.4697295285901464Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA84320.4280513763181794Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT73820.3747480147036054No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG69890.35479732792786484No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAAGTATC66090.33550658753401896TruSeq Adapter, Index 3 (97% over 36bp)
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG65970.33489740625842385No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG62630.3179418607543593No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG51950.2637247272263926No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT51830.26311554595079745No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48610.24676918172232806No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA45790.23245342174584246No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG43900.22285881665521914No Hit
AGAACGTAGCGGTTTTACCGTTCGTCCGGTTGCAGGTTATCTGAGATCGG43660.22164045410402888No Hit
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA39490.20047140477709802No Hit
TTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAA39360.1998114583952033No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA35090.17813475800527653Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA34960.1774748116233818No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC32920.16711872993826457No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT31520.16001161505632136Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC31090.15782871548543878Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA30580.1552396950641595No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC29810.15133078187909071TruSeq Adapter, Index 5 (97% over 35bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCACAC28270.14351295550895318No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT27880.141533116363269Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGGCATCTGTATGAACATACCGATAATCTGTGAGATCGGAAGAGCACA27220.1381826193474958No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC26900.13655813594590877No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA26890.13650737083960918No Hit
AACGTAGCGGTTTTACCGTTCGTCCGGTTGCAGGTTATCTGAGATCGGAA26770.13589818956401403No Hit
ATCATGATCGGCATCTGTATGAACATACCGATAATCTGTGAGATCGGAAG26560.13483212233172256No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAAGA24140.12254696660722073No Hit
ATGTGCAGAAACTGGCAACCTGTTATTTCTAGATCGGAAGAGCACACGTC24000.12183625511902639No Hit
TTCTGCGTCGCGAAACTCTGGAAGATGAGATCGGAAGAGCACACGTCTGA23510.11934876491034628Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGATCGGCATCTGTATGAACATACCGATAATCTGTGAGATCGGAAGAGCA23490.1192472346977471No Hit
ACGTAGCGGTTTTACCGTTCGTCCGGTTGCAGGTTATCTGAGATCGGAAG22600.1147291402370832No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT22150.11244471045360147No Hit
ACTCCATCCTGATCAAATTCAACCAGATCGGTTCTCTGACCGAAACTCTG21990.11163246875280795No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA21570.10950033428822499No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAAGTATCTCGTATG21490.10909421343782823TruSeq Adapter, Index 3 (97% over 36bp)
TCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGAA21350.1083835019496339Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC21010.1066574883354477Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAGATCGGA20730.10523606535905905No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTAGATCGGAAGAGCACAC20670.10493147472126149No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC20540.10427152833936677No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA20110.10208862876848421Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)