FastQCFastQC Report
Fri 14 Jul 2023
SRR9691612_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691612_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2048075
Total Bases155.6 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA612872.992419711192217No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG328471.6037986890128535No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC250341.2223185186089376Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA184130.8990393418209782No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC181740.8873698472956313No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA174400.8515313159918461Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA148740.726242935439376Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC136770.6677978101387889No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA111740.5455854888126656No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA108910.5317676354625686No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG107570.5252249063144661No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA92570.45198540092525913Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG87090.4252285682897355No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATCATC73990.36126606691649477TruSeq Adapter, Index 7 (97% over 42bp)
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT65650.3205449019200957Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA65100.3178594533891581No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG60980.2977430025755893No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA53440.2609279445332813No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG52800.25780305897000844No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA48430.23646594973328613No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA47060.22977674157440525Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG45200.22069504290614358No Hit
AAACTGCGACGACACATTCACAGATCGGAAGAGCACACGTCTGAACTCCA44980.21962086349376855Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACTGCGACGACACATTCACCAGATCGGAAGAGCACACGTCTGAACTCC44130.2154706248550468Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC43480.21229691295484784Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA42800.20897672204387047Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT42650.20824432698997838Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT42500.2075119319360863No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT40450.19750253286622807No Hit
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGAGATCGGA38080.18593069101473333No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA36640.17889969849736947Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG36610.17875321948659106No Hit
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT36500.17821612978040355Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAA36400.17772786641114216No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC35050.17113631092611356Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAACTGCGACGACACATTCAGATCGGAAGAGCACACGTCTGAACTCCAGT34180.16688841961353956Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAAACTGCGACGACACATTCACAGATCGGAAGAGCACACGTCTGAACTCC33970.16586306653809066Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAA33120.16171282789936892No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG31970.15609779915286306No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCAGATCGGAAGAGCACACGT30000.14647901077841388No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT29760.14530717869218657No Hit
AAAACTGCGACGACACATTCACCAGATCGGAAGAGCACACGTCTGAACTC28000.1367137433931863Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC27980.136616090719334No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC26750.13061045127741905Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA26100.12743673937722008No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC25990.12689964967103257Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATCATCTCGTATG24480.11952687279518572TruSeq Adapter, Index 7 (97% over 49bp)
AAAACTGCGACGACACATTCAGATCGGAAGAGCACACGTCTGAACTCCAG24060.11747616664428792Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCA23760.1160113765365038No Hit
AACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAG23710.1157672448518731No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG23350.11400949672253212No Hit
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGA23110.11283766463630483No Hit
TGTGGGCACTCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTG23000.1123005749301173TruSeq Adapter, Index 7 (97% over 36bp)
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC22720.11093343749618544No Hit
AAAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGAGATCGG22530.11000573709458882No Hit
TGCGTCTGGATCGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC22050.10766207292213421Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA21900.10692967786824212No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGA21340.10419540300037841Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAAGA21050.1027794392295204No Hit
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGTAGATCGG20990.10248648120796358No Hit
GGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAG20960.10234000219718517No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)