FastQCFastQC Report
Fri 14 Jul 2023
SRR9691615_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691615_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2713631
Total Bases206.2 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA418271.5413665306742148No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC350251.2907060687322631Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA346991.2786926446521285Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG269870.9944977780693101No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG261600.9640220059396432No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC259890.957720485946689No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCTAATC165320.6092206346404503TruSeq Adapter, Index 10 (97% over 38bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA113540.4184061871345073No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA105420.388483179916503Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC102840.37897562343590563No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT91930.3387711888609763No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA91260.3363021722555498Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA75450.27804075056630767No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA74450.2743556511552234No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG69210.25504573024114185No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA67170.24752812744252994No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT66370.24458004791366256No Hit
AAACTGCGACGACACATTCACAGATCGGAAGAGCACACGTCTGAACTCCA66300.24432209095488666Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA60530.22305906735293046Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACTGCGACGACACATTCAGATCGGAAGAGCACACGTCTGAACTCCAGT59360.21874750104196186Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA55090.20301212655663203Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT54610.20124327883931162Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC54260.1999534940454321Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA54160.19958498410432368No Hit
TGTGGGCACTCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAG53160.19589988469323943TruSeq Adapter, Index 7 (97% over 36bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG53020.19538397077568764No Hit
AAACTGCGACGACACATTCACCAGATCGGAAGAGCACACGTCTGAACTCC50740.1869819441184155Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC49620.18285463277800112Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAACTGCGACGACACATTCACAGATCGGAAGAGCACACGTCTGAACTCC48170.17751123863192897Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT47080.17349448027384712No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG46870.17272060939751943No Hit
TGCGTCTGGATCGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC46490.1713202716213074Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCTAATCTCGTATG43160.1590488905823968TruSeq Adapter, Index 10 (97% over 38bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA41910.15444251631854147No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA41410.15259996661299932Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAA40530.14935707913124519TruSeq Adapter, Index 7 (97% over 35bp)
AAAACTGCGACGACACATTCAGATCGGAAGAGCACACGTCTGAACTCCAG37470.13808067493332735Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACGTAGCGGTTTTACCGTTCGTCCGGTTGCAGGTTATCTGAGATCGGAA37110.13675403914533701No Hit
ACGTAGCGGTTTTACCGTTCGTCCGGTTGCAGGTTATCTGAGATCGGAAG35900.13229506885792505No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT35760.13177915494037326Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCTAAT35120.12942069131727932TruSeq Adapter, Index 10 (97% over 38bp)
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA33570.1237087872300987No Hit
ATGTGCAGAAACTGGCAACCTGTTATTTCTAGATCGGAAGAGCACACGTC33550.12363508524187704No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG33090.12193993951277826No Hit
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCTAATCT33000.12160828056578069TruSeq Adapter, Index 10 (97% over 38bp)
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGAGATCGGA32190.11862335004280244No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG31960.11777577717825305No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT31130.1147171446670531No Hit
ATCGGCATCTGTATGAACATACCGATAATCTGTGAGATCGGAAGAGCACA30310.111695363149964No Hit
TGTGGGCACTCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA30160.11114259823830137Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC29850.11000021742086526No Hit
AAAACTGCGACGACACATTCACCAGATCGGAAGAGCACACGTCTGAACTC29660.10930004853275924Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC29290.10793656175065806No Hit
TTCCGCAGCTGGAAGATGTGAGCATGTTTCTGAGATCGGAAGAGCACACG28880.1064256709921135No Hit
TCCGTGTATGGACTGACGAAGAATCAGATCGGAAGAGCACACGTCTGAAC28280.10421461134546296Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT28110.10358814444557864Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGA27660.10192984971059071Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)