FastQCFastQC Report
Fri 14 Jul 2023
SRR9691616_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691616_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2808841
Total Bases213.4 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA426581.5187046899415098No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA352621.255393238705929Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC352241.254040367539494Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG276960.9860294690941922No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG268150.9546642191565846No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC264360.941171109365037No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCTAATC169240.6025260952827163TruSeq Adapter, Index 10 (97% over 38bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA115450.4110236214865847No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA111280.3961776405286024Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC106050.37755786105372285No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT96100.3421339976168106No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA92510.32935292528128146Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA78850.28072076703522914No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA75870.2701114089405559No Hit
AAACTGCGACGACACATTCACAGATCGGAAGAGCACACGTCTGAACTCCA70050.24939111897042235Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA68830.24504769048871045No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT68150.24262676313824813No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA61740.21980596267286043Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACTGCGACGACACATTCAGATCGGAAGAGCACACGTCTGAACTCCAGT59610.21222276376626517Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC57380.20428354613166072Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTGGGCACTCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAG56550.2013285906891846TruSeq Adapter, Index 7 (97% over 36bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG55640.1980888202643012No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA55220.19659354160666268No Hit
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA54820.195169466694626Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT54620.19445742923860768Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC52690.18758626778803072Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA51180.1822103849950923No Hit
AAACTGCGACGACACATTCACCAGATCGGAAGAGCACACGTCTGAACTCC50380.17936223517101893Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAACTGCGACGACACATTCACAGATCGGAAGAGCACACGTCTGAACTCC48500.1726690830844466Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG47890.17049736884359065No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT47590.16942931265956315No Hit
TGCGTCTGGATCGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC47440.1688952845675494Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCTAATCTCGTATG44360.157929907744867TruSeq Adapter, Index 10 (97% over 38bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA41220.14675091968537915Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAA41200.1466797159397773TruSeq Adapter, Index 7 (97% over 35bp)
AACGTAGCGGTTTTACCGTTCGTCCGGTTGCAGGTTATCTGAGATCGGAA38330.13646197844591415No Hit
AAAACTGCGACGACACATTCAGATCGGAAGAGCACACGTCTGAACTCCAG38140.13578554286269676Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT37000.13172692936339223Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTAGCGGTTTTACCGTTCGTCCGGTTGCAGGTTATCTGAGATCGGAAG36830.13112169752577665No Hit
ACGGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCTAAT35900.1278107233552914TruSeq Adapter, Index 10 (97% over 38bp)
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA35440.1261730372064492No Hit
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCTAATCT34450.12264845179915844TruSeq Adapter, Index 10 (97% over 38bp)
ATGTGCAGAAACTGGCAACCTGTTATTTCTAGATCGGAAGAGCACACGTC34200.12175840497913551No Hit
AGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGT34190.1217228031063346No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG33080.11777099522543284No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG32640.1162045128221925No Hit
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGAGATCGGA32060.11413960419973933No Hit
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC31720.11292914052450816No Hit
TGTGGGCACTCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA31680.11278673303330448Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCGGCATCTGTATGAACATACCGATAATCTGTGAGATCGGAAGAGCACA30550.10876372140680088No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT30400.10822969331478711No Hit
AAAACTGCGACGACACATTCACCAGATCGGAAGAGCACACGTCTGAACTC30060.10701922963955596Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCCGCAGCTGGAAGATGTGAGCATGTTTCTGAGATCGGAAGAGCACACG29910.1064852015475422No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC29690.10570196034592202No Hit
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT28770.10242658804823768Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGAGATCGGAAGAGCACACGTC28390.10107371688180283No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA28210.10043288317138635No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)