FastQCFastQC Report
Fri 14 Jul 2023
SRR9691617_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691617_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2784682
Total Bases211.6 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA422091.5157565567630344No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA349881.2564450806232095Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC348221.2504838972636731Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG275750.9902387418024751No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG264260.9489772979464083No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC263500.9462480814685483No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCTAATC169390.6082920778746012TruSeq Adapter, Index 10 (97% over 38bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA115960.4164209773324207No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA110450.39663415786793604Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC104690.37594956982520805No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA92000.3303788367935728Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT90410.32466902863594477No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA81830.2938576110306311No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA76120.2733525767035518No Hit
AAACTGCGACGACACATTCACAGATCGGAAGAGCACACGTCTGAACTCCA70320.25252434568830484Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA69140.24828687799899593No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT66060.2372263691150372No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA60290.21650587032917942Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC57870.20781547049178326Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAACTGCGACGACACATTCAGATCGGAAGAGCACACGTCTGAACTCCAGT57370.20601993333529645Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA54540.1958571930295811No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG54500.1957135500570622No Hit
TGTGGGCACTCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAG54340.19513897816698642TruSeq Adapter, Index 7 (97% over 36bp)
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA53990.19388210215744564Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT51740.18580218495325498Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC51710.18569445272386578Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA51640.18544307752195763No Hit
AAACTGCGACGACACATTCACCAGATCGGAAGAGCACACGTCTGAACTCC49670.1783686611253996Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAACTGCGACGACACATTCACAGATCGGAAGAGCACACGTCTGAACTCC49120.1763935702532641Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG48020.17244338850899313No Hit
TGCGTCTGGATCGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC46810.16809818859029504Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT46780.16799045636090584No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCTAATCTCGTATG43620.1566426615319092TruSeq Adapter, Index 10 (97% over 38bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA41950.15064556742924326Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAA40430.14518713447352335TruSeq Adapter, Index 7 (97% over 35bp)
AAAACTGCGACGACACATTCAGATCGGAAGAGCACACGTCTGAACTCCAG38510.13829227179261402Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT36710.1318283380292615Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGTAGCGGTTTTACCGTTCGTCCGGTTGCAGGTTATCTGAGATCGGAA36500.13107421242353703No Hit
ACGGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCTAAT36040.12942231823956918TruSeq Adapter, Index 10 (97% over 38bp)
ACGTAGCGGTTTTACCGTTCGTCCGGTTGCAGGTTATCTGAGATCGGAAG35710.12823726371628788No Hit
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCTAATCT34960.12554395798155768TruSeq Adapter, Index 10 (97% over 38bp)
AGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGT34780.12489756460522243No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG33590.12062418617278382No Hit
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGAGATCGGA33510.12033690022774594No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA33310.1196186853651512No Hit
ATGTGCAGAAACTGGCAACCTGTTATTTCTAGATCGGAAGAGCACACGTC32770.11767950523614545No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG32440.11649445071286416No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT31050.11150285741783084No Hit
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC30870.11085646404149559No Hit
ATCGGCATCTGTATGAACATACCGATAATCTGTGAGATCGGAAGAGCACA30680.1101741599220306No Hit
TGTGGGCACTCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA30020.107804050875468Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCCGCAGCTGGAAGATGTGAGCATGTTTCTGAGATCGGAAGAGCACACG29800.10701401452661381No Hit
AAAACTGCGACGACACATTCACCAGATCGGAAGAGCACACGTCTGAACTC29470.10582896000333251Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC29460.10579304926020279No Hit
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT28800.1034229402136402Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGTGTATGGACTGACGAAGAATCAGATCGGAAGAGCACACGTCTGAAC28580.10263290386478599Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)