FastQCFastQC Report
Fri 14 Jul 2023
SRR9691618_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691618_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2638815
Total Bases200.5 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA406451.5402747066391542No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC341501.29414149911987Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA328751.2458243567662Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG260320.9865034115692081No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG251910.954633045514748No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC248490.9416726826245873No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCTAATC156340.5924629047508067TruSeq Adapter, Index 10 (97% over 38bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA109250.4140115923245851No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA102450.388242449735961Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC102170.3871813673940765No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA88390.33496095785418833Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT88270.33450620827909494No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA74400.28194473655788677No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA71850.27228130808715273No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT66120.25056701587644453No Hit
AAACTGCGACGACACATTCACAGATCGGAAGAGCACACGTCTGAACTCCA64940.2460953117213598Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA62570.23711400761326581No Hit
AAACTGCGACGACACATTCAGATCGGAAGAGCACACGTCTGAACTCCAGT57800.2190377120033045Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA57360.21737029689462883Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA54160.20524364155880573No Hit
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA53860.20410676762107233Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC53770.2037657054397523Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53580.20304568527918782No Hit
TGTGGGCACTCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAG53100.20122668697881435TruSeq Adapter, Index 7 (97% over 36bp)
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT51500.1951633593109028Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG50300.19061586355996915No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC47550.1801945191307462Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAACTGCGACGACACATTCACCAGATCGGAAGAGCACACGTCTGAACTCC47130.1786028956179194Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT46300.1754575443901903No Hit
AAAACTGCGACGACACATTCACAGATCGGAAGAGCACACGTCTGAACTCC45610.17284273433340344Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGCGTCTGGATCGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC43980.16666571927171855Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG43670.1654909495360607No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCTAATCTCGTATG42010.15920024708060249TruSeq Adapter, Index 10 (97% over 38bp)
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAA39890.15116633792061968TruSeq Adapter, Index 7 (97% over 35bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA39840.15097685893099744Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA37360.14157870104573456No Hit
AAAACTGCGACGACACATTCAGATCGGAAGAGCACACGTCTGAACTCCAG37350.1415408052478101Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACGTAGCGGTTTTACCGTTCGTCCGGTTGCAGGTTATCTGAGATCGGAA36470.13820597503045876No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT35550.1347195616214096Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCTAAT35520.13460587422763629TruSeq Adapter, Index 10 (97% over 38bp)
ACGTAGCGGTTTTACCGTTCGTCCGGTTGCAGGTTATCTGAGATCGGAAG34750.13168789778745382No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG32660.12376767602124439No Hit
ATGTGCAGAAACTGGCAACCTGTTATTTCTAGATCGGAAGAGCACACGTC31960.12111497016653308No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA31870.12077390798521305No Hit
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCTAATCT31730.1202433668142708TruSeq Adapter, Index 10 (97% over 38bp)
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGAGATCGGA31730.1202433668142708No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG31490.11933386766408408No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT30410.11524112148824377No Hit
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC29370.11129995850410128No Hit
ATCGGCATCTGTATGAACATACCGATAATCTGTGAGATCGGAAGAGCACA29340.11118627111032793No Hit
AAAACTGCGACGACACATTCACCAGATCGGAAGAGCACACGTCTGAACTC29210.1106936257373101Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTGGGCACTCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA28980.10982202238504783Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCCGCAGCTGGAAGATGTGAGCATGTTTCTGAGATCGGAAGAGCACACG28960.10974623078919894No Hit
TCCGTGTATGGACTGACGAAGAATCAGATCGGAAGAGCACACGTCTGAAC27780.10527452663411417Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT27610.10463029806939857Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC27450.10402396530260744No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)