FastQCFastQC Report
Fri 14 Jul 2023
SRR9691619_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691619_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2393578
Total Bases181.9 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG465281.9438681338147326No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC255671.0681498576607906No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG190710.7967569889094903No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA160470.6704189293183677No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGCCATC124620.5206431543070666TruSeq Adapter, Index 20 (97% over 39bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA109590.4578501306412409No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA107930.45091490647056415No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC92540.3866178582857964Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA92450.38624185215606094Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT79570.3324311971450272No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA75150.31396511832912904No Hit
AAACTGCGACGACACATTCAGATCGGAAGAGCACACGTCTGAACTCCAGT74050.30936948785458424Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA65220.2724791086816473Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT65000.27155998258673836Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC58740.24540666734069247Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA53560.2237654256514724No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG47910.20016059639585593No Hit
ATCGGCATCTGTATGAACATACCGATAATCTGTGAGATCGGAAGAGCACA43820.18307320672232114No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC43350.18110961915592472No Hit
ATCCCGTCGGAAGCACTGACAGCGGTTTCTGAGATCGGAAGAGCACACGT42690.17835224087119783No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA41650.17400728114981004Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAACTGCGACGACACATTCAGATCGGAAGAGCACACGTCTGAACTCCAG41410.17300459813718208Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT41180.17204369358341362TruSeq Adapter, Index 18 (100% over 35bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA39510.1650666909538774No Hit
AAACTGCGACGACACATTCACAGATCGGAAGAGCACACGTCTGAACTCCA38960.162768875716605Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGACTGACTAAGCATGTAGTACCGAGGAGATCGGAAGAGCACACGTCTG37660.15733767606487026Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT36110.15086201494164803No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGCCATCTCGTATG35470.14818819357464014TruSeq Adapter, Index 20 (97% over 39bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC32760.13686623122371613No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA32550.13598888358766664No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC31330.13089191160680788Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGTGTATGGACTGACGAAGAATCAGATCGGAAGAGCACACGTCTGAAC29870.12479225661332115Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG29840.12466692123674265Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC29420.12291222596464371No Hit
AGACGATCCGGTACGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA29180.12190954295201577Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC28940.12090685993938781No Hit
AAGACTGACTAAGCATGTAGTACCGAGGATGAGATCGGAAGAGCACACGT28840.12048907535079283No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT28780.12023840459763585No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA28550.11927750004386739No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT28540.11923572158500789Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG28030.11710502018317348No Hit
TGCGTCTGGATCGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC28010.11702146326545448Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGAGTATGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTA27250.11384630039213262TruSeq Adapter, Index 3 (97% over 36bp)
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG26490.11067113751881076No Hit
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGCCATCT25460.10636795625628244TruSeq Adapter, Index 20 (97% over 39bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT25400.10611728550312545No Hit
AACTGCGACGACACATTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC25260.10553238707909247Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGAGCA25180.10519815940821647No Hit
ACGGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGCCAT25100.1048639317373405TruSeq Adapter, Index 20 (97% over 39bp)
ATTTCAATCCGTATACACAGAGCATCGAGATCGGAAGAGCACACGTCTGA24880.10394480564243154Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTGATCCACGTAGATGTAAACTAGATCGGAAGAGCACACGTCTGAACTC24480.10227366728805161Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAAACTGCGACGACACATTCACAGATCGGAAGAGCACACGTCTGAACTCC24160.10093675660454768Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGACTGACTAAGCATGTAGTACCGAGATCGGAAGAGCACACGTCTGAAC24060.1005189720159527Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)