FastQCFastQC Report
Fri 14 Jul 2023
SRR9691622_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691622_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2340755
Total Bases177.8 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG455291.9450561891355567No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC246801.0543606656826536No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG192140.8208462654143642No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA161060.6880685932530316No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGCCATC119160.5090665191359198TruSeq Adapter, Index 20 (97% over 39bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA109250.4667297517254049No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA106670.45570766697069964No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC93550.39965737550491187Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA92130.3935909567639501Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT78040.3333967032004631No Hit
AAACTGCGACGACACATTCAGATCGGAAGAGCACACGTCTGAACTCCAGT72590.3101136171876168Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA72240.30861837313174595No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA66060.2822166352309404Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT63820.2726470732733669Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC56940.2432548472608197Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55190.2357786269814654No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG46480.1985684106196505No Hit
ATCGGCATCTGTATGAACATACCGATAATCTGTGAGATCGGAAGAGCACA45480.19429628474573374No Hit
ATCCCGTCGGAAGCACTGACAGCGGTTTCTGAGATCGGAAGAGCACACGT43580.18617924558529195No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC43460.18566659048042192No Hit
AAAACTGCGACGACACATTCAGATCGGAAGAGCACACGTCTGAACTCCAG42320.18079636698415683Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA41120.17566981593545672Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA40550.17323470418732417No Hit
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT40350.17238027901254083TruSeq Adapter, Index 18 (100% over 35bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA39460.16857808698475493No Hit
AAACTGCGACGACACATTCACAGATCGGAAGAGCACACGTCTGAACTCCA37700.16105914544666144Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT36800.15721423216013636No Hit
AAGACTGACTAAGCATGTAGTACCGAGGAGATCGGAAGAGCACACGTCTG36140.1543946290833513Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGCCATCTCGTATG33400.1426890041888194TruSeq Adapter, Index 20 (97% over 39bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC32340.13816055076246767No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA30960.13226501705646254No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC30470.13017167537824334Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGTGTATGGACTGACGAAGAATCAGATCGGAAGAGCACACGTCTGAAC29820.12739479356019748Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG29790.12726662978397996Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT29400.12560050069315243No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC29400.12560050069315243No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT28860.12329355272123738Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC28440.12149925985419235No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA27970.11949136069345147No Hit
TGCGTCTGGATCGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC27770.11863693551866811Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGACTGACTAAGCATGTAGTACCGAGGATGAGATCGGAAGAGCACACGT27320.11671447887540559No Hit
AGGGAGTATGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTA26830.11462113719718638TruSeq Adapter, Index 3 (97% over 36bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG26760.11432208838601221No Hit
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG25920.11073350265192214No Hit
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGCCATCT25700.10979363495966046TruSeq Adapter, Index 20 (97% over 39bp)
AACTGCGACGACACATTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC24650.10530790279204785Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGA24610.1051370177570912Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGAGCA24570.10496613272213452No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT24280.10372721621869865No Hit
ACGGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGCCAT24240.103556331183742TruSeq Adapter, Index 20 (97% over 39bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG23940.10227469342156698No Hit
ACGCGATCGTGGCGATTACCGAACATATGTGAGATCGGAAGAGCACACGT23650.10103577691813112No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)