FastQCFastQC Report
Fri 14 Jul 2023
SRR9691625_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691625_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2321380
Total Bases176.4 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG768373.3099707932350584No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA264561.1396669222617581No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC144560.6227330294910786No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG118440.5102137521646607No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC99900.4303474657315907Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC97890.4216888230276818Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA82040.35341047135755455No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACAAGATC77500.33385313908106384TruSeq Adapter, Index 7 (97% over 35bp)
TTCAGACTCTGAACGCTAAAGTTGAGATCGGAAGAGCACACGTCTGAACT75400.32480679595757694Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA67560.2910337816298926No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA65080.2803504811792985Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC58000.24985138150582842No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC52780.22736475717030388No Hit
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT51550.2220661847694044Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC50710.21844764752000967Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48940.21082287260164212No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT44770.192859419827861No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA42780.18428693277274724No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA40870.17605906831281393No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA38240.16472960049625654Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACAAGATCTCGTATG36350.15658789168511836TruSeq Adapter, Index 7 (97% over 35bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGAAC34080.14680922554687298Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGAAAGCTACTAAACTGGTACTGGGCGAGATCGGAAGAGCACACGTCTG33130.14271683222910508Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA32210.1387536723845299TruSeq Adapter, Index 10 (100% over 35bp)
ACACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT32010.13789211589657877Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCAGACTCTGAACGCTAAAGAGATCGGAAGAGCACACGTCTGAACTCCA31640.13629823639386918Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTAGAGGAAGAAAAAATGACAATATTGAATCAGATCGGAAGAGCACACG30800.13267969914447442No Hit
AGCTGACCAGACAGTCGCCGCTTCGTCGTCGTCCTCTAGATCGGAAGAGC30510.13143044223694528No Hit
AGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGAGCA29860.1286303836511041No Hit
AGGGAGTATGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAC29310.12626110330923848TruSeq Adapter, Index 7 (97% over 35bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAGATCGGAAGAGCACACGTC29280.1261318698360458No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA28000.12061790831315855No Hit
AGCTGACCAGACAGTCGCCGCTTCGTCGTCGTCCTCAGATCGGAAGAGCA27980.12053175266436343No Hit
ACTGCGCGGCTATATGACAGATATGAAACGAGATCGGAAGAGCACACGTC26020.11208849908244234No Hit
ATCTAGAGGGTATTAATAATGAAAGCTACTAAACAGATCGGAAGAGCACA25960.111830032136057No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG25330.10911612919901094No Hit
TAATTAGAGGAAGAAAAAATGACAATATTGAATCAGATCGGAAGAGCACA25130.10825457271105979No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGAGATCGGAAGAGCACACGTC24300.1046791132860626No Hit
TGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGGAAGAGCACACG24130.10394679027130413No Hit
ACGGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACAAGAT23630.1017928990514263TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)