FastQCFastQC Report
Fri 14 Jul 2023
SRR9691629_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691629_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1808048
Total Bases137.4 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC623733.449742484712795Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA268131.482980540339637Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA237321.3125757723246285No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG192441.0643522738334381No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC158800.8782952664973497No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT141280.7813951841986496Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA124440.688256063998301Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT98600.5453395042609489Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC87390.48333893790430343No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG78700.43527605461801905No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA73210.40491181650044694No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG72990.4036950346450979No Hit
ACGTAGCGGTTTTACCGTTCGTCCGGTTGCAGGTTATCTGAGATCGGAAG69080.38206950257957756No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA66500.3677999699123032No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA63570.35159464792970097No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA60550.33489155155172873Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC59550.32936072493650614No Hit
AGAACGTAGCGGTTTTACCGTTCGTCCGGTTGCAGGTTATCTGAGATCGG52780.2919170287514491No Hit
TGTGGGCACTCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTC52500.29036839729918673TruSeq Adapter, Index 1 (97% over 36bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG50640.2800810597948727No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC49130.2717295116058866Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA44740.24744918276505937Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACGTAGCGGTTTTACCGTTCGTCCGGTTGCAGGTTATCTGAGATCGGAA43370.23987195030220437No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG42360.23428581542082952No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC42080.2327371839685672No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC41840.23140978558091377Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41400.22897622187021582No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT38360.2121625089599391No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT38330.21199658416148243TruSeq Adapter, Index 12 (100% over 35bp)
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA38160.2110563436368946No Hit
ATGTGCAGAAACTGGCAACCTGTTATTTCTAGATCGGAAGAGCACACGTC37620.2080696972646744No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA37000.20464058476323638Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTACATC36800.20353441944019188TruSeq Adapter, Index 1 (97% over 36bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG36650.20270479544790845No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT36620.20253887064945178No Hit
ATTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACT36460.2016539383910162Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC34950.19330239020203002No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA31010.17151093333805298Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA30600.1692432944258117No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA30360.1679158960381583No Hit
TTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTCC29800.16481863313363362Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGTGATTTTCTGGCAGGTCTGGCAGATCGGAAGAGCACACGTCTGAACTC29060.1607258214383689Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG28420.15718609240462642No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA28350.15679893454156082No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA27370.1513787244586427No Hit
TTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTC27360.15132341619249048Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG27030.14949824340946702Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC25310.13998522163128413Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTCCGGAACCGGATACCTGTCATGAACAGATCGGAAGAGCACACGTCTG23960.1325186057007336Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTCCA23630.13069343291771013Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTGGGCACTCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC23620.13063812465155794Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA23260.12864702707007777No Hit
TTTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACT23230.12848110227162113Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG22480.12433298231020415No Hit
ACGGACGTGGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC22270.1231715087210074Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTACAT21940.12134633593798394TruSeq Adapter, Index 1 (97% over 36bp)
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC21630.11963177968726493No Hit
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA21040.1163685919842836No Hit
TTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACTC20810.1150965018627824Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGAGATCGGA20170.11155677282903993No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGA20030.11078245710290878Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC19960.11039529923984319No Hit
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG19730.10912320911834199No Hit
TGTGGGCACTCGAAGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC19590.10834889339221083Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCAGATCGGAAGAGCACACGT19590.10834889339221083No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC19020.10519632222153394No Hit
AAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTC18790.10392423210003274No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT18360.10154597665548702Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGACGTGGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC18210.10071635266320363Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)