FastQCFastQC Report
Fri 14 Jul 2023
SRR9691632_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691632_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2631489
Total Bases199.9 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA370841.4092401678289364No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG304191.155961510764438No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTACATCTCGTATG250230.9509065019842379TruSeq Adapter, Index 1 (97% over 36bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA170750.6488721784510595No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC162950.6192311653212307Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA135690.5156396245623676Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA114450.4349248657319107Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC94570.3593782835497317Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA91920.3493079393453668No Hit
TTCCGCAGCTGGAAGATGTTAGCAACTTTCTGAGATCGGAAGAGCACACG81810.3108886261732426No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC80660.3065184768015371No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG74750.2840597091608591No Hit
AACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAG74190.2819316364233329No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG68920.26190495191125635No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA64960.24685643755303555Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC63370.24081423103041663No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT61500.2337079881390346Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG52220.1984427827743152No Hit
AGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGAGCA48650.1848763190725859No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC45380.17244989433738844Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA44430.16883977094337085Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAGATCGGAAGA44050.1673957215857638No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA41770.15873142544012153No Hit
ATTCCGCAGCTGGAAGATGTTAGCAACTTTCTGAGATCGGAAGAGCACAC39290.14930710331679137No Hit
AGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACAC39270.14923110071902257No Hit
ACAACGTCGTCGTACTGGGCACCCAATGGGGTGACGAGATCGGAAGAGCA38100.14478494874954825No Hit
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT35120.1334605616819983TruSeq Adapter, Index 12 (100% over 35bp)
TTCCGCAGCTGGAAGATGTTAGCAACTTTCTGAAAGAGATCGGAAGAGCA32910.1250622746285468No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTAGATCGGAAGAGCACAC32900.1250242733296624No Hit
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG32670.1241502434553213No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC32630.12399823825978372Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA31730.1205781213601881No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCAGATCGGAAGAGCACACGT30720.11673999017286411No Hit
ATGCCGTGTTCGTCTGACTAACGGTTTCGAAGTGAGATCGGAAGAGCACA30230.11487792652752872No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTAGATCGGAAGAGCAC30100.11438390964203157No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGATCGGAAGAGCA29620.11255984729558056No Hit
AGCACTTCACTCTGAAGTCTGACGTTCTGAGATCGGAAGAGCACACGTCT27850.10583361739304249Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA27720.10533960050754536No Hit
ATATCTTCAGGAGATCGTCATGGACAGATTCCCACGAGATCGGAAGAGCA26900.10222349399902489No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)