FastQCFastQC Report
Fri 14 Jul 2023
SRR9691633_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691633_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2636411
Total Bases200.3 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA382511.4508739342993182No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG322391.222836651796704No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTACATCTCGTATG253540.9616861710863746TruSeq Adapter, Index 1 (97% over 36bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA175470.6655639048691574No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC166210.630440397950092Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA141610.5371317294609983Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA115870.43949900072484904Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC94730.35931423438909943Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA92990.352714352959383No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC83450.31652879615507595No Hit
TTCCGCAGCTGGAAGATGTTAGCAACTTTCTGAGATCGGAAGAGCACACG82500.31292541261586304No Hit
AACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAG76770.2911913203214522No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG71930.2728330294479882No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG68920.26141599318163977No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA65800.24958172303180345Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC65200.24730590184914264No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT62010.23520611922799592Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG51240.19435512899923418No Hit
AGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGAGCA50220.19048623298871079No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC46460.1762244202440363Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA46260.17546581318314938Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAGATCGGAAGA45930.17421411153268593No Hit
ATTCCGCAGCTGGAAGATGTTAGCAACTTTCTGAGATCGGAAGAGCACAC40310.1528972531217629No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA39920.15141796935303334No Hit
AGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACAC39390.14940766064168295No Hit
ACAACGTCGTCGTACTGGGCACCCAATGGGGTGACGAGATCGGAAGAGCA38820.14724563051815517No Hit
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT34830.1321114196534607TruSeq Adapter, Index 12 (100% over 35bp)
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG34300.13010111094211033No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTAGATCGGAAGAGCACAC33750.12801494152467122No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC33590.12740805587596168Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCCGCAGCTGGAAGATGTTAGCAACTTTCTGAAAGAGATCGGAAGAGCA32700.12403225445501478No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA32630.12376674198370437No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCAGATCGGAAGAGCACACGT31540.11963233350187052No Hit
ATGCCGTGTTCGTCTGACTAACGGTTTCGAAGTGAGATCGGAAGAGCACA31440.11925302997142706No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTAGATCGGAAGAGCAC30810.11686341772963321No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGATCGGAAGAGCA29950.11360140736781936No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA29510.11193247183386809No Hit
AGCACTTCACTCTGAAGTCTGACGTTCTGAGATCGGAAGAGCACACGTCT28360.10757048123376817Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATATCTTCAGGAGATCGTCATGGACAGATTCCCACGAGATCGGAAGAGCA27640.1048394958145752No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG27300.1035498638110674No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG26400.10013613203707616No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)