FastQCFastQC Report
Fri 14 Jul 2023
SRR9691636_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691636_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3403854
Total Bases258.6 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA492291.4462723724343052No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA365451.0736359432572606No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG319890.9397876642182655No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC301520.8858194270377049No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG215670.633605319147061No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA209720.6161251334516697No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA207700.6101906838542429Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC172750.5075129544334158Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA153730.4516351171348712Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG135510.3981075569046146No Hit
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA132860.390322264115911Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC130290.38277199903403614Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC125800.3695810689882703No Hit
TTCCGCAGCTGGAAGATGTTAGCAACTTTCTGAGATCGGAAGAGCACACG113990.3348851037676704No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG105610.3102659514773548No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA102850.3021574955917616No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGGACATCTCGTATG102270.3004535447172529TruSeq Adapter, Index 20 (97% over 37bp)
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG82430.24216667342371323No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA81530.23952261172188938No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG80920.23773052545731985No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA67930.19956790156099527No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA65270.19175323030893804Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG64330.18899165475369978No Hit
AACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAG63230.18576002378480394No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC61280.1800312234308522Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT56330.1654888840708209Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACAC55980.16446063785344495No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT55740.16375555473295858No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCAGATCGGAAGAGCACACGT55610.16337363470936178No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTAGATCGGAAGAGCAC55120.16193409000503547No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG54650.16055330222741634No Hit
ATTCCGCAGCTGGAAGATGTTAGCAACTTTCTGAGATCGGAAGAGCACAC53570.15738042818522768No Hit
ACCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAGATCGGAAGA49080.1441894981394619No Hit
ACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCA48230.14169232875440604No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGAGATCGGAAGA47470.13945956553953254No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC46870.13769685773831664Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGAGATCGGAAGAGCA46030.13522906681661434No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGA43680.1283251279285187No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCACAC43540.1279138294415683No Hit
ACAACGTCGTCGTACTGGGCACCCAATGGGGTGACGAGATCGGAAGAGCA43080.12656242012730276No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA41790.12277259835468855Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAA41670.12242005679444536No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA39230.1152517117361673No Hit
AAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACA38820.11404719473866977No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTAGATCGGAAGAGC38770.11390030242190176No Hit
GGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAG38650.11354776086165858No Hit
GATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCA38190.11219635154739303No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTAGATCGGAAGAGCACAC38150.11207883769397864No Hit
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC35350.10385286795497105TruSeq Adapter, Index 6 (100% over 35bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA35070.10303027098107027Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGA34880.10247208017735192Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG34550.10150259088668315No Hit
TCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACA34390.10103253547302557No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCA34040.10000428925564962No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)