FastQCFastQC Report
Fri 14 Jul 2023
SRR9691637_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691637_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3576066
Total Bases271.7 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA514021.4373895783802648No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA387981.084935233298267No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG342690.9582876826098848No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC317940.8890775505821201No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG221440.6192279448981087No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA221120.6183331068274467Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA219450.6136631706461794No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC182440.5101695550361767Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA160520.4488731471958291Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG144350.4036558609376896No Hit
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC140300.3923305666058736Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA139650.3905129267748414Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC133100.3721967100159785No Hit
TTCCGCAGCTGGAAGATGTTAGCAACTTTCTGAGATCGGAAGAGCACACG121500.3397588299544807No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG110760.3097258272078871No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA108210.30259508633229926No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGGACATCTCGTATG101470.2837475594689807TruSeq Adapter, Index 20 (97% over 37bp)
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG86840.2428366814259021No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA85990.24045976780070616No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG85190.2382226726240511No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA71210.1991294344120047No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG67800.1895938162215127No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA67710.18934214301413899Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAG66670.1864339192844875No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC65260.18249103903563302Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT61190.17110981732440061No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTAGATCGGAAGAGCAC58370.16322405682669167No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCAGATCGGAAGAGCACACGT58050.16232921875602965No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG57580.16101492533974485No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT57490.16076325213237116Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCCGCAGCTGGAAGATGTTAGCAACTTTCTGAGATCGGAAGAGCACAC57440.16062343368383022No Hit
AGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACAC57040.1595048860955027No Hit
ACCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAGATCGGAAGA53870.1506403964580072No Hit
ACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCA51190.1431461276162129No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC50260.14054550447335146Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGAGATCGGAAGA49730.13906342891881748No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGAGATCGGAAGAGCA48070.13442145642725833No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCACAC46320.1295278107283255No Hit
ACAACGTCGTCGTACTGGGCACCCAATGGGGTGACGAGATCGGAAGAGCA45300.12667551437809035No Hit
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAA45050.12597642213538565No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGA44300.12387914540727156No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA42970.1201599746760826Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTAGATCGGAAGAGCACAC40540.11336479807699298No Hit
GATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCA40430.11305719749020293No Hit
GGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAG40250.11255385107545553No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTAGATCGGAAGAGC40030.11193864990187541No Hit
AAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACA38770.10841522499864377No Hit
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC37500.10486383640570393TruSeq Adapter, Index 6 (100% over 35bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCA37040.10357750667912728No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA36730.10271063229817347No Hit
TCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACA36610.10237506802167522No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG36550.10220728588342608No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGA36350.10164801208926233Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACT35820.10016593653472838Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)