FastQCFastQC Report
Fri 14 Jul 2023
SRR9691639_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691639_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2266253
Total Bases172.2 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC1267185.591520452482579Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA448391.9785522622584504Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA316221.395342885370698No Hit
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT227031.0017857670789625Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG213250.9409805524802395No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC186010.8207821456827635No Hit
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA182860.8068825501830554Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC180930.7983662900832343No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT113550.501047323489478Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG112350.4957522394895892No Hit
ACGTAGCGGTTTTACCGTTCGTCCGGTTGCAGGTTATCTGAGATCGGAAG90570.39964646489160743No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA82110.36231612269239133No Hit
AACGTAGCGGTTTTACCGTTCGTCCGGTTGCAGGTTATCTGAGATCGGAA79930.35269672009259334No Hit
TTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTCC78150.3448423454927583Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC77730.3429890660927972Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG74900.3305014929930595No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA72270.3188964338933032Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA70900.3128512129934301No Hit
TTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTC70880.312762961593432Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGAACGTAGCGGTTTTACCGTTCGTCCGGTTGCAGGTTATCTGAGATCGG66760.2945831731938138No Hit
TGTGGGCACTCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCG66150.2918915054938703TruSeq Adapter, Index 16 (97% over 36bp)
TCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTCCA61260.2703140381943234Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC58340.257429333794594No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG58090.25632619129461715No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGGACATCTCGTATG57250.252619632494695TruSeq Adapter, Index 20 (97% over 37bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA57080.2518694955947107Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA57010.2515606156947172No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA54540.24066156779494607No Hit
TTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACGTCTGAAC53900.2378375229950054Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACGTCTGAACT52480.231571673595137Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGGACATC51450.22702672649523242TruSeq Adapter, Index 20 (97% over 37bp)
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT51100.22548232699526488No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG50480.22274653359532232Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT45090.1989627812958218No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG43580.19229980059596172No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG42940.18947575579602102Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT41790.18440130029612758No Hit
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA40160.1772088111962786No Hit
TGTGGGCACTCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG38730.17089883609641113Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTGGGCACTCGAAGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC36110.1593379026966539Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC36020.15894077139666224No Hit
TCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACGTCTGA35540.15682273779670672Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG31480.13890770359708293No Hit
ATGTGCAGAAACTGGCAACCTGTTATTTCTAGATCGGAAGAGCACACGTC30990.13674554429712835No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA28490.12571411929736No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA27680.12213993759743506No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA27580.12169868059744432Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC26790.11821275029751752TruSeq Adapter, Index 6 (100% over 35bp)
AGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGT26590.11733023629753607No Hit
ACGGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGGACAT26580.11728611059753699TruSeq Adapter, Index 20 (97% over 37bp)
CGTGATTTTCTGGCAGGTCTGGCAGATCGGAAGAGCACACGTCTGAACTC26310.11609471669756201Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC25410.11212340369764541No Hit
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA25270.11150564389765837Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCGCAGCTGGAAGATGTGAGCATGTTTCTGAGATCGGAAGAGCACACG25080.11066725559767598No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA24830.10956411309769915No Hit
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGAGATCGGA23990.10585755429777699No Hit
TGCGTCTGGATCGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC23780.10493091459779645Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCGTGTATGGACTGACGAAGAATCAGATCGGAAGAGCACACGTCTGAAC23710.10462203469780294Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG23490.10365126929782333No Hit
ACTCCGGAACCGGATACCTGTCATGAACAGATCGGAAGAGCACACGTCTG23470.10356301789782518Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGAA22820.10069484739788541Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACA22720.10025359039789468No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)