FastQCFastQC Report
Fri 14 Jul 2023
SRR9691645_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691645_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3751439
Total Bases285.1 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA854262.277152847214096No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA838742.235782055899083Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC734821.9587683552897968No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG410241.093553700326728No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG302970.8076100930869461No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG250550.6678770466479663No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC243520.6491375709427769No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA241560.6439129091529944No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA240860.6420469585137863No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA238580.6359692907175087Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA220130.5867881631555251Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC175620.4681403589395962No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA171070.45601167978474394No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA151320.403365215321374Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCACAC121450.3237424359025963No Hit
GATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCA119410.31830452261118997No Hit
AACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGG117200.3124134498788332No Hit
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAA114040.30399001556469396No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAGATCGGA112620.3002048014108719No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG103140.2749344984684544No Hit
ACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGGA101820.2714158486916621No Hit
TGTGGGCACTCGAAGATACGGATTCTTAAGATCGGAAGAGCACACGTCTG99740.26587130964944383Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT99270.26461845707740417No Hit
ACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCA90120.24022781657918468No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC88420.23569622216967945Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA84550.2253801807786292No Hit
ATGGCGTCGGCAAGACCCGTTCTAAAGCCATCCTGGCTGCAGCGGGTATC83000.22124843293466856No Hit
ATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGA78820.21010604197482619No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCA76840.20482806730963773No Hit
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA69990.18656840748310183Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAGATCGGAA66860.17822494248207155No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG65990.17590583240191296No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT64970.17318687575620983No Hit
TCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACA60850.16220442342258531No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA60590.16151135604230804Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGA60570.1614580431669021No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT60410.16103154016365454Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGACTGGATGGATTCCAGTAACCCCGGAACCTGGACGGATGAGATCGGA58120.15492721592967393No Hit
ACCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAGATCGGAAGA57690.15378098910844612No Hit
AGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCA56620.15092875027422809No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC56450.15047559083327758Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAG55420.14772997774987146No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTACATCTCGTATG54300.14474445672713857TruSeq Adapter, Index 1 (97% over 36bp)
AACGTAGCGGTTTTACCGTTCGTCCGGTTGCAGGTTATCTGAGATCGGAA53730.14322503977806916No Hit
ACGTAGCGGTTTTACCGTTCGTCCGGTTGCAGGTTATCTGAGATCGGAAG52600.1402128623176333No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG51980.13856016318004905No Hit
TTCTGGCTGAACAACTGGAAGCTTTCGTTGACTTACGAGATCGGAAGAGC50440.13445507177379132No Hit
AACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAG49490.131922710192009No Hit
AAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAA45940.12245967480745389No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA43950.11715504370456245No Hit
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG42540.11339648598844337No Hit
ATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAGATCGGAA41620.11094409371976993No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC40530.10803854201014598Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGA39580.10550618042836361No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG38230.10190756133846238No Hit
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGA37870.10094792958115538No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)