FastQCFastQC Report
Fri 14 Jul 2023
SRR9691646_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691646_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3498288
Total Bases265.8 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA794542.27122523931706No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA751132.1471359705090034Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC682211.9501253184414777No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG385991.103368276139643No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG276850.7913871013478593No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG236870.677102628485705No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC220420.6300796275206615No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA214280.6125281852151682Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA212990.6088406672063592No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA210340.6012655333122945No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA206770.5910605416134979Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA162050.46322658397479No Hit
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC160750.4595104805550601No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA135440.38716080551401144Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCA120190.34356805385948785No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCACAC113410.3241871452550505No Hit
AACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGG110790.3166977675937487No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAGATCGGA103010.29445831789721144No Hit
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAA102920.2942010491989225No Hit
ACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGGA95470.2729049180627781No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG95400.27270482018633113No Hit
TGTGGGCACTCGAAGATACGGATTCTTAAGATCGGAAGAGCACACGTCTG91070.26032733725753854Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT90840.25966987280635556No Hit
ACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCA82270.2351721756470594No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC78150.22339498634760774Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA77600.22182278874695283No Hit
ATGGCGTCGGCAAGACCCGTTCTAAAGCCATCCTGGCTGCAGCGGGTATC76510.21870697895656388No Hit
ATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGA72700.20781593739566326No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCA69710.1992688995302845No Hit
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA66010.18869229748951485Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT60300.1723700278536244No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG58280.1665957748475826No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAGATCGGAA57820.16528084594521664No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGA56600.16179342581285475No Hit
TCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACA56020.16013547197943678No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT54700.15636219773786492Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGACTGGATGGATTCCAGTAACCCCGGAACCTGGACGGATGAGATCGGA54470.15570473328668194No Hit
GGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAG54060.15453273143892096No Hit
AGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCA52050.14878706384380017No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA51940.1484726243236692Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAGATCGGAAGA51710.1478151598724862No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC51440.14704335377761923Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACGTAGCGGTTTTACCGTTCGTCCGGTTGCAGGTTATCTGAGATCGGAA49460.1413834424152614No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTACATCTCGTATG48480.1385820721450035TruSeq Adapter, Index 1 (97% over 36bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG47920.13698128913342755No Hit
ACGTAGCGGTTTTACCGTTCGTCCGGTTGCAGGTTATCTGAGATCGGAAG46320.13240762338606768No Hit
AAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAA43840.12531844147765991No Hit
TTCTGGCTGAACAACTGGAAGCTTTCGTTGACTTACGAGATCGGAAGAGC43220.12354614600055798No Hit
AACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAG42170.12054467785385309No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA41700.11920116354056612No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC39390.11259793361781535Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG38680.11056836944242442No Hit
ATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAGATCGGAA38580.11028251533321443No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGA37260.10650924109164253No Hit
GGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCAC36110.10322191883572764No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG36040.10302182095928065No Hit
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGA35260.10079215890744272No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)