FastQCFastQC Report
Fri 14 Jul 2023
SRR9691648_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691648_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14761680
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA3120862.11416315758098No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA2239061.5168056752347971No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG1585521.074078289192016No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC1094140.741202898315097No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG975230.6606497363443727No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC912170.6179310214013581No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA687370.46564483175356736No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA685980.46470320451330743Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG680340.46088250117872764No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG612370.41483760655968693No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA585010.3963031308089594Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA572980.388153651887861No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA546330.37010015120230216Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT542350.3674039811186803Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG523250.354465074435972No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA517320.3504479164973093No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG475780.32230748803659204No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC449250.30433527891134343Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG402350.2725638274234369No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGGACATCTCGTATG393220.26637889454316854TruSeq Adapter, Index 20 (97% over 37bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG371450.2516312506435582No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG343260.23253450826735167No Hit
TTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAA313750.2125435587277329No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA309150.2094273822491749No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTAGATCGGAAGAGCAC298700.20234824220549422No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG292670.19826334129990625No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT282390.1912993642999984No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCACAC262850.17806238856281942No Hit
AGAACGTAGCGGTTTTACCGTTCGTCCGGTTGCAGGTTATCTGAGATCGG251080.17008904135572644No Hit
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC250570.16974355222440807No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGAGATCGGAAGAGCA238570.1616143961933872No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC229190.15526010589580588No Hit
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA228900.1550636512917229No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGA228550.1548265509074848No Hit
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA226410.15337685141528606No Hit
ACGTAGCGGTTTTACCGTTCGTCCGGTTGCAGGTTATCTGAGATCGGAAG215200.14578286482297406No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTAGATCGGAAGAGCACAC212720.14410283924322975No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGAGATCGGAAGA209320.14179957836777385No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA207180.14034987887557515No Hit
GGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAG204540.13856146454875054No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA203020.13753177145148793Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG202750.13734886544078995No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC200310.13569593704781568Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA199590.13520818768595444Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAA196340.1330065412608863No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA195180.13222072284455427No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTAGATCGGAAGAGCACA194610.13183458793308078No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG193460.13105554381344128No Hit
TTCCGCAGCTGGAAGATGTGAGCATGTTTCTGAGATCGGAAGAGCACACG187720.12716709751193633No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC182990.1239628551763756TruSeq Adapter, Index 6 (100% over 35bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG178190.12071119276396725No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA176950.11987117997409509No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC175510.11889568125037259No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC172740.11701920106654527Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA170970.1158201505519697No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG167190.11325946640219813Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG164750.11160653800922388No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTAGATCGGAAGAGC162410.11002135258317482No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGA161990.10973683212208908No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA160770.10891036792560196Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCAGATCGGAAGAGCACACGT159150.10781293186141415No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAAGA158660.1074809913234808No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)