FastQCFastQC Report
Fri 14 Jul 2023
SRR9691651_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691651_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1460910
Total Bases111 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA275871.8883435666810413No Hit
ATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGAGATCGGAAGAGC249311.7065390749601277No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC215231.4732598175110034No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA199241.3638074898522154No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG160141.096166088260057No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT159241.0900055444893937Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC151021.0337392447173337Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG149921.0262096912198562No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA123550.8457057587394158Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA121390.8309204536898235Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG121060.8286615876405801No Hit
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA111460.762949120753503No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG109830.751791691479968No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC95890.6563717135210246No Hit
ATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGTTCCAGGAGATCG92130.6306343306569193No Hit
ATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGTAGATCGGAAGAG74970.5131732960962688No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA73170.5008522085549418No Hit
AATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGAGATCGGAAGAG65670.4495143437994127No Hit
ACGCCGCCAGCGGTCGTCAGACTGTCGATGAGATCGGAAGAGCACACGTC64770.4433538000287492No Hit
TGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGTTCCAGGAGATCGGAA59530.40748574518621955No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT55680.38113230794504793No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG51980.35580562799898696No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA51630.35340986097706223No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG47640.32609811692712076No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA46090.3154882915443114No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG45590.31206576722727614No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA44830.30686353026538254No Hit
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA39140.26791520353752113Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG37630.25757918010007463No Hit
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG37540.2569631257230082No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA36040.24669555277190247No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA33100.22657110978773504Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAA32690.22376463984776612No Hit
ATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCAGATCGGAAGAGCA32200.22041056601707154No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA31570.21609818537760714No Hit
ATGAAAACGGCAAGTACGACATTAAAGACGAGATCGGAAGAGCACACGTC31470.21541368051420004No Hit
ATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGTTAGATCGGAAGA28650.19611064336612113No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC28440.1946731831529663No Hit
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC27550.1885810898686435No Hit
AAGACTGACTAAGCATGTAGTACCGAGGATGAGATCGGAAGAGCACACGT27340.1871436296554887No Hit
TCATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAA26810.1835157538794313No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTAAATC26100.1786557693492412TruSeq Adapter, Index 6 (97% over 38bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA25770.17639690329999794No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC23910.16366511284062674No Hit
ACTGCGGTGATCAACGCCGCCAGCGGTCGTCAGACTGTCGATGAAGCCCT23890.16352821186794533No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC23090.15805217296068888No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG21600.14785305049592376No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT21230.14532038250131765No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA21220.14525193201497696Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACTGCGGTGATCAACGCCGCCAGCGGTCGTCAGACTGTCGATGAAGCCC21210.14518348152863628No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC20910.1431299669384151Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGTTCCAGATCGGAA20790.14230856110232665No Hit
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT20630.14121335332087534Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGTTCAGATCGGAAG20590.1409395513755125No Hit
ATCTGGCCTGTTGGCTGAAATCACCCCGGACAAGATCGGAAGAGCACACG20500.14032349699844618No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20190.1382015319218843No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC20120.13772237851749938No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT18880.1292345182112519Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG18260.12499058805812814No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT18200.12457988514008392No Hit
ACACATGAATGCAGACACCGATTACTCCAAGATCGGAAGAGCACACGTCT18090.12382692979033616Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA17880.12238946957718136No Hit
AAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTC17770.12163651422743359No Hit
ATGGTGTAACGGTACTGCCGACCTTCAAGGGTCAACAGATCGGAAGAGCA17350.11876159380112396No Hit
TTTAATAAAGGCGAAACAGCGATGACCAGATCGGAAGAGCACACGTCTGA17340.11869314331478326Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCA17130.11725568310162844No Hit
AACCTGGGAAGAGATCCCGGCGCTGGATAAAGAACTGAGATCGGAAGAGC16940.11595512386115504No Hit
TTCTGCGTCGCGAAACTCTGGAAGATGCAGATCGGAAGAGCACACGTCTG16190.11082133738560213Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGTCTCGCTGAAGTCGGTAAGAAATTCGAGAAGATCGGAAGAGCACAC16080.11006838203585435No Hit
TAAAGACTGACTAAGCATGTAGTACCGAGGATGAGATCGGAAGAGCACAC16000.10952077814512873No Hit
AACGCCGCCAGCGGTCGTCAGACTGTCGATGAGATCGGAAGAGCACACGT15940.10911007522708448No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA15360.10513994701932357No Hit
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGAGATCGGA15310.10479769458762005No Hit
AAATCACCCCGGACAAAGCGTTCCAGGACAAGCTGAGATCGGAAGAGCAC14780.10116981881156265No Hit
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT14710.1006906654071777Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)