FastQCFastQC Report
Fri 14 Jul 2023
SRR9691653_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691653_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1541335
Total Bases117.1 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA291201.8892713135042025No Hit
ATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGAGATCGGAAGAGC264471.7158502207501938No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC222961.446538228224234No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA213021.382048678580581No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG167631.0875637028939198No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT165041.0707600878459258Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG157841.0240473355889537No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC153910.9985499583153565Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG130580.8471876652382513No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA128760.83537971952885Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA127900.829800140787045Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA121890.7908079684169891No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG116250.7542163124823611No Hit
ATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGTTCCAGGAGATCG98800.6410027670817829No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC96330.6249776979047384No Hit
ATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGTAGATCGGAAGAG80680.5234423405684033No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA76320.4951551739239036No Hit
AATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGAGATCGGAAGAG69230.449156088715302No Hit
ACGCCGCCAGCGGTCGTCAGACTGTCGATGAGATCGGAAGAGCACACGTC66580.43196320073183314No Hit
TGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGTTCCAGGAGATCGGAA61090.3963447271358919No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT59770.38778072255544704No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG56010.363386285265695No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA55550.3604018594270551No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG49710.3225126270408445No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA49300.31985259531510024No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA49100.31855501886351767No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG48700.3159598659603525No Hit
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA40270.26126701852614775Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG39550.2565957433004506No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG38750.25140543749412037No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA37560.24368485760720413No Hit
TTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAA35780.2321364271881194No Hit
ATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCAGATCGGAAGAGCA33360.21643575212397045No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA33070.21455426626917576Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA32660.21189423454343154No Hit
ATGAAAACGGCAAGTACGACATTAAAGACGAGATCGGAAGAGCACACGTC31890.20689856520483868No Hit
ATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGTTAGATCGGAAGA31870.2067688075596804No Hit
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC30260.19632331712444084No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC30180.1958042865438078No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTAAATC28420.1843856137698813TruSeq Adapter, Index 6 (97% over 38bp)
TCATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAA28140.1825690067376657No Hit
AAGACTGACTAAGCATGTAGTACCGAGGATGAGATCGGAAGAGCACACGT28020.18179046086671619No Hit
ACTGCGGTGATCAACGCCGCCAGCGGTCGTCAGACTGTCGATGAAGCCCT26650.1729020621733757No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA26270.17043666691536882No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC25350.16446781523808907No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC24620.15973166118981275No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA23260.15090814131905134Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT22540.14623686609335412No Hit
ATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGTTCAGATCGGAAG22450.145652956690142No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG21890.14201974262571085No Hit
ATCTGGCCTGTTGGCTGAAATCACCCCGGACAAGATCGGAAGAGCACACG21680.14065728735154914No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC21390.13877580149675445No Hit
ATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGTTCCAGATCGGAA21350.1385162862064379No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC21210.13760798269033014Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TACTGCGGTGATCAACGCCGCCAGCGGTCGTCAGACTGTCGATGAAGCCC21210.13760798269033014No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT20960.13598601212585193Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA20920.13572649683553542No Hit
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT20210.13112010043241734Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG19830.1286547051744105No Hit
ACACATGAATGCAGACACCGATTACTCCAAGATCGGAAGAGCACACGTCT19750.12813567459377748Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACCTGGGAAGAGATCCCGGCGCTGGATAAAGAACTGAGATCGGAAGAGC19530.12670834049703664No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT19390.12580003698092887No Hit
AAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTC18380.11924727590043695No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA17700.11483551596505627No Hit
ATGGTGTAACGGTACTGCCGACCTTCAAGGGTCAACAGATCGGAAGAGCA17650.11451112185216063No Hit
TTTAATAAAGGCGAAACAGCGATGACCAGATCGGAAGAGCACACGTCTGA17610.11425160656184412Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGTCTCGCTGAAGTCGGTAAGAAATTCGAGAAGATCGGAAGAGCACAC17130.11113742307804599No Hit
AACGCCGCCAGCGGTCGTCAGACTGTCGATGAGATCGGAAGAGCACACGT16960.1100344830942008No Hit
AGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCA16810.10906130075551389No Hit
TTCTGCGTCGCGAAACTCTGGAAGATGCAGATCGGAAGAGCACACGTCTG16700.10834763370714348Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGAGATCGGA16360.10614175373945313No Hit
TAAAGACTGACTAAGCATGTAGTACCGAGGATGAGATCGGAAGAGCACAC15700.1018597514492307No Hit
AAATCACCCCGGACAAAGCGTTCCAGGACAAGCTGAGATCGGAAGAGCAC15680.10172999380407245No Hit
AAAGACGCGCAGACTTAACAGTAAAGGAGATCGGAAGAGCACACGTCTGA15590.10114608440086029Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)