FastQCFastQC Report
Fri 14 Jul 2023
SRR9691657_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691657_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2411430
Total Bases183.2 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGAGATCGGAAGAGC430921.7869894626839675No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA291371.2082871988819912No Hit
ACGCCGCCAGCGGTCGTCAGACTGTCGATGAGATCGGAAGAGCACACGTC264661.0975230464910863No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA214750.8905504202900354No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT213070.8835835997727489Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG186450.7731926699095557No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC177890.7376950606071916No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC176080.7301891408832104Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGTAGATCGGAAGAG147340.6110067470339177No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA140320.5818953898723994Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA113320.46992863155886755Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG112730.46748195054386815No Hit
AATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGAGATCGGAAGAG107500.44579357476683956No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG104850.4348042447842152No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC88490.36696068308016405No Hit
ATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGTTCCAGGAGATCG86960.3606159001090639No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA81340.337310226711951No Hit
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA81080.3362320282985614No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA77390.32092990466237875No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG74780.3101064513587373No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA73970.3067474486093314No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC70540.29252352338653825No Hit
ATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCAGATCGGAAGAGCA69260.28721546965908196No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT68930.2858469870574721No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG68640.2846443811348453No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA68380.28356618272145573No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG63960.2652368096938331No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT59400.24632686828976996No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG59030.24479250900917715No Hit
AACGCCGCCAGCGGTCGTCAGACTGTCGATGAGATCGGAAGAGCACACGT54060.22418233164553814No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG51480.213483285851134No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG48840.20253542503825533No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTCAATC48440.2008766582484252TruSeq Adapter, Index 10 (97% over 36bp)
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA47990.19901054560986634Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG47050.19511244365376562No Hit
TGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGTTCCAGGAGATCGGAA45690.1894726365683433No Hit
ATCTGGCCTGTTGGCTGAAATCACCCCGGACAAGATCGGAAGAGCACACG43960.1822984702023281No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA43780.18155202514690455Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGTTAGATCGGAAGA42980.17823449156724433No Hit
TACTGCGGTGATCAACGCCGCCAGCGGTCGTCAGACTGTCGATGAAGCCC41270.17114326354072065No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT40660.16861364418622973No Hit
ATGAAAACGGCAAGTACGACATTAAAGACGAGATCGGAAGAGCACACGTC40330.1672451615846199No Hit
ATCCCGTCGGAAGCACTGACAGCGGTTTCTGAGATCGGAAGAGCACACGT39040.16189563868741785No Hit
ACGCCGCCAGCGGTCGTCAGACTGTCGATGAAGCAGATCGGAAGAGCACA38990.16168829283868907No Hit
AACTATCTGCTGACTGATGAAGGTCTGGAGATCGGAAGAGCACACGTCTG38840.1610662552925028Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTGCGGTGATCAACGCCGCCAGCGGTCGTCAGACTGTCGATGAAGCCCT38500.1596563035211472No Hit
ATCGCTGTTGAAGCGTTATCGCTGATTTATAACAGATCGGAAGAGCACAC38320.15890985846572367No Hit
TTCTGCGTCGCGAAACTCTGGAAGATGCAGATCGGAAGAGCACACGTCTG37740.15650464662047Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGTCTCGCTGAAGTCGGTAAGAAGATCGGAAGAGCACACGTCTGAACT36240.15028427115860715Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC34400.14265394392538866No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA33850.14037313958937228No Hit
ATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGTTCAGATCGGAAG33740.139916978722169No Hit
TTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAA33700.139751102043186No Hit
AAACTATCTGCTGACTGATGAAGGTCTGGAGATCGGAAGAGCACACGTCT33180.13759470521640685Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA33100.13726295185844084No Hit
AAAACTATCTGCTGACTGATGAAGGTCTGGAGATCGGAAGAGCACACGTC32490.13473333250394995No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA31530.1307522922083577Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT30940.1283056111933583Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGCGGTGATCAACGCCGCCAGCGGTCGTCAGACTGTCGATGAGATCGG30910.12818120368412103No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA30420.12614921436657917Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCGTACTGCGGTGATCAACGCCGCCAGCGGTCGTCAGACTGAGATCGGA30230.12536130014140986No Hit
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA29910.12403428670954579No Hit
AATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGTAGATCGGAAGA28910.11988736973497054No Hit
AACCTGGGAAGAGATCCCGGCGCTGGATAAAGAACTGAGATCGGAAGAGC28880.1197629622257333No Hit
ACTATCTGCTGACTGATGAAGGTCTGGAGATCGGAAGAGCACACGTCTGA28040.11627955196709006Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA27560.11428903181929394No Hit
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGAGATCGGA27240.11296201838742986No Hit
TTTAATAAAGGCGAAACAGCGATGACCAGATCGGAAGAGCACACGTCTGA26720.11080562156065073Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATCACCCCGGACAAAGCGTTCCAGGACAAGCTGAGATCGGAAGAGCAC26500.10989329982624418No Hit
ACGCCGCCAGCGGTCGTCAGACTGAGATCGGAAGAGCACACGTCTGAACT26500.10989329982624418Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGTTCCAGATCGGAA26010.1078613105087023No Hit
TGCCGTGCGTACTGCGGTGATCAACGCCGCCAGCGGTCGTCAGACTGAGA25740.106741642925567No Hit
ACGCCGCCAGCGGTCGTCAGACTGTCGAGATCGGAAGAGCACACGTCTGA25460.10558050617268593Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)