FastQCFastQC Report
Fri 14 Jul 2023
SRR9691658_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691658_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2293286
Total Bases174.2 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGAGATCGGAAGAGC411061.7924497860275606No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA271191.1825389419374646No Hit
ACGCCGCCAGCGGTCGTCAGACTGTCGATGAGATCGGAAGAGCACACGTC254451.1095432492938082No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT204910.893521348841793Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA202310.8821839055399109No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG178620.7788823548393005No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC171120.7461781914684866No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC170570.743779886154627Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGTAGATCGGAAGAG137970.6016257893694898No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA137040.5975704731115089Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA107250.4676695362026367Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG106920.4662305530143209No Hit
AATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGAGATCGGAAGAG103340.45061976569865253No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG102600.44739295491273223No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC84760.3696006516413565No Hit
ATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGTTCCAGGAGATCG82450.3595277693231459No Hit
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA78620.34282684322845036No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA74330.32412006178034486No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA73260.3194542678061088No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA70700.30829124670887104No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG69410.3026661306090911No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG68120.2970410145093111No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC67450.2941194425815184No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT67300.2934653593141021No Hit
ATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCAGATCGGAAGAGCA66720.29093623734675916No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA63300.2760231388496681No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG58910.2568803018899518No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT56790.24763592504380175No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG54790.2389148148115848No Hit
AACGCCGCCAGCGGTCGTCAGACTGTCGATGAGATCGGAAGAGCACACGT51050.22260633867733898No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG48840.2129695118707392No Hit
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA46440.2025041795920788Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG46160.2012832241595684No Hit
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG45930.20028029648286347No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTCAATC45760.199539002113125TruSeq Adapter, Index 10 (97% over 36bp)
TGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGTTCCAGGAGATCGGAA44790.19530926365049975No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA42080.18349215928584572Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41880.182620048262624No Hit
ATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGTTAGATCGGAAGA41060.17904439306741504No Hit
ATCTGGCCTGTTGGCTGAAATCACCCCGGACAAGATCGGAAGAGCACACG39900.1739861491327292No Hit
ATGAAAACGGCAAGTACGACATTAAAGACGAGATCGGAAGAGCACACGTC39280.17128260496074194No Hit
TACTGCGGTGATCAACGCCGCCAGCGGTCGTCAGACTGTCGATGAAGCCC38980.16997443842590937No Hit
TTCTGCGTCGCGAAACTCTGGAAGATGCAGATCGGAAGAGCACACGTCTG37800.16482898338890134Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT37750.1646109556330959No Hit
ATCCCGTCGGAAGCACTGACAGCGGTTTCTGAGATCGGAAGAGCACACGT37520.16360802795639096No Hit
ACGCCGCCAGCGGTCGTCAGACTGTCGATGAAGCAGATCGGAAGAGCACA37450.16330278909826335No Hit
ATCGCTGTTGAAGCGTTATCGCTGATTTATAACAGATCGGAAGAGCACAC36330.15841896736822184No Hit
ACTGCGGTGATCAACGCCGCCAGCGGTCGTCAGACTGTCGATGAAGCCCT35680.15558460654275133No Hit
AACTATCTGCTGACTGATGAAGGTCTGGAGATCGGAAGAGCACACGTCTG35300.1539275955986301Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGTCTCGCTGAAGTCGGTAAGAAGATCGGAAGAGCACACGTCTGAACT34480.15035194040342112Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC34230.14926180162439398No Hit
AAACTATCTGCTGACTGATGAAGGTCTGGAGATCGGAAGAGCACACGTCT33200.14477042985480224Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGTTCAGATCGGAAG31950.13931973595966662No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA31940.13927613040850553No Hit
AAAACTATCTGCTGACTGATGAAGGTCTGGAGATCGGAAGAGCACACGTC31500.1373574861574178No Hit
TTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAA30740.13404346426917532No Hit
TGCGTACTGCGGTGATCAACGCCGCCAGCGGTCGTCAGACTGAGATCGGA29800.12994454246003334No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA29610.1291160369879727Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT29450.12841834816939535Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA28740.12532235403695832No Hit
ACTGCGGTGATCAACGCCGCCAGCGGTCGTCAGACTGTCGATGAGATCGG28380.12375255419515925No Hit
AATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGTAGATCGGAAGA27660.12061295451156114No Hit
AACCTGGGAAGAGATCCCGGCGCTGGATAAAGAACTGAGATCGGAAGAGC27350.1192611824255675No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA27030.11786580478841278Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTAATAAAGGCGAAACAGCGATGACCAGATCGGAAGAGCACACGTCTGA26900.11729893262331868Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTATCTGCTGACTGATGAAGGTCTGGAGATCGGAAGAGCACACGTCTGA25660.11189184427934415Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGAGATCGGA25540.11136857766541111No Hit
ACGCCGCCAGCGGTCGTCAGACTGTCGAGATCGGAAGAGCACACGTCTGA25450.11097612770496136Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA25270.11019122778406182No Hit
ACGCCGCCAGCGGTCGTCAGACTGAGATCGGAAGAGCACACGTCTGAACT24800.10814176687949083Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGTTCCAGATCGGAA24340.10613591152608091No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA24300.10596148932143656No Hit
TGCCGTGCGTACTGCGGTGATCAACGCCGCCAGCGGTCGTCAGACTGAGA24260.10578706711679223No Hit
AAATCACCCCGGACAAAGCGTTCCAGGACAAGCTGAGATCGGAAGAGCAC24060.10491495609357053No Hit
ACGGTCTCGCTGAAGTCGGTAAGAAATTCGAGAAGATCGGAAGAGCACAC23280.1015137231030059No Hit
AAGACTGACTAAGCATGTAGTACCGAGGATGAGATCGGAAGAGCACACGT23180.10107766759139505No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)