FastQCFastQC Report
Tue 18 Jul 2023
SRR9691660_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691660_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4373709
Total Bases332.4 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA812361.8573709407736088Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC511571.1696480035594503No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA399850.9142126282292672No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA336680.7697814372195315Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG328150.750278539335836No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC315090.7204182994341873No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT313430.7166228937499043No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG305810.6992006098256651No Hit
ATATCATTCTGACCTGTAGCGTTCGAGATCGGAAGAGCACACGTCTGAAC284440.6503404776129368Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA249550.5705683665739993No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA194910.4456400734479592Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG180860.4135163084695392No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA168310.38482212694077267No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC160860.3677885291408276No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGAGATCGGAAGAGCACACGTC152390.34842281459511826No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG135840.31058307720060935No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG117170.26789619519725705Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC116570.2665243618173957Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTGATATCTCGTATG114650.2621344950018394TruSeq Adapter, Index 12 (97% over 37bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC97820.22365456869672856Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA91410.2089988154218765No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC84510.19322273155347097Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA82750.18919868697254436Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT82210.18796403693066915Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA78170.1787270255062694No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT78130.17863556994761198No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA74710.17081611968240226Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG74170.16958146964052706TruSeq Adapter, Index 2 (100% over 35bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA72750.16633479730818854No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA72550.16587751951490143No Hit
TGTGGGCACTCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC67930.15531440248996903Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCGTGTATGGACTGACGAAGAATCAGATCGGAAGAGCACACGTCTGAAC67780.15497144414500372Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGACTGGATGGATTCCAGTAACCCCGGAACCTGGACGGATGAGATCGGA67030.153256652420177No Hit
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC66010.15092453567441272No Hit
TGTGGGCACTCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGT64260.14692335498315046TruSeq Adapter, Index 22 (100% over 36bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA62170.14214480204330007No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA59860.1368632435308339No Hit
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA58940.13475976568171316Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCGTCTGGATCGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC57870.13231332948762708Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCATTCTGACCTGTAGCGTTCGTACCCAGATCGGAAGAGCACACGTCTGA55320.12648303762321636Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAGATCGGAAGAGCACA55190.12618580705757973No Hit
AGAACGGCGGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG55110.1260028959402649TruSeq Adapter, Index 2 (100% over 35bp)
TGTGGGCACTCGAAGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC54400.12437955977409561Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC54290.12412805698778771Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC52920.12099570410377097Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGAACGGCGGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC51500.11774903177143245Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACA50780.11610283171559882No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC48950.1119187399070217Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG48740.11143859822407022No Hit
ACAACGTCGTCGTACTGGGCACCCAATGGGGTGACGAGATCGGAAGAGCA45820.10476234244207834No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT45810.10473947855241397Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGACGGGGATCAAGAGAGGAGATCGGAAGAGCACACGTCTGAACTCCAG45010.10291036737926551Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT44500.10174430900638336Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)