FastQCFastQC Report
Fri 14 Jul 2023
SRR9691661_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691661_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4424405
Total Bases336.2 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA834501.8861293213437738Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC520971.1774916627207501No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA409080.9245989008691564No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA342890.7749968639851008Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG329310.7443034713142219No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC318920.7208200876728057No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG316090.7144237473739407No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT315700.7135422729157932No Hit
ATATCATTCTGACCTGTAGCGTTCGAGATCGGAAGAGCACACGTCTGAAC288100.6511610035699716Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA254240.5746309390754237No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA199100.45000401183888Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG187800.4244638544617864No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA169990.3842098542063848No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC164710.37227604615761895No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGAGATCGGAAGAGCACACGTC151930.3433908062214015No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG135980.3073407610740879No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG122600.2770994065868744Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC120250.2717879579288062Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTGATATCTCGTATG115600.2612780701585863TruSeq Adapter, Index 12 (97% over 37bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC100280.22665194528981863Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA95870.21668450333999714No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC88970.20108918600354173Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA84350.19064710396087156Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT82980.18755064240276376Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA80710.18242000901816177No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT80410.1817419517426637No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA75650.17098344297142778Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG74800.16906228069084997TruSeq Adapter, Index 2 (100% over 35bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA74180.1676609623214873No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA72910.16479051985521218No Hit
TCCGTGTATGGACTGACGAAGAATCAGATCGGAAGAGCACACGTCTGAAC71720.1621008926624032Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGGCACTCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC71250.16103860293078956Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGACTGGATGGATTCCAGTAACCCCGGAACCTGGACGGATGAGATCGGA67630.1528567118064463No Hit
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC66340.14994106552180464No Hit
TGTGGGCACTCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGT65750.14860755287999178TruSeq Adapter, Index 22 (100% over 36bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA63160.14275365840152518No Hit
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA60110.13586007610062822Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA59840.13524982455267995No Hit
TGCGTCTGGATCGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC58360.13190474199355617Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAGATCGGAAGAGCACA56860.1285144556160659No Hit
TGTGGGCACTCGAAGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC56790.128356242251783Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGAACGGCGGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG55950.12645768188038845TruSeq Adapter, Index 2 (100% over 35bp)
TCATTCTGACCTGTAGCGTTCGTACCCAGATCGGAAGAGCACACGTCTGA54080.12223112486311719Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC53590.12112363131313703Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACA53340.1205585835835553No Hit
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC52900.11956409957949149Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGAACGGCGGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC51680.1168066666591327Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG50540.11423004901224007No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC48870.11045553017863419Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGACGGGGATCAAGAGAGGAGATCGGAAGAGCACACGTCTGAACTCCAG47030.10629677888891273Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT46230.10448862615425125Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT45670.1032229192399882Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACAACGTCGTCGTACTGGGCACCCAATGGGGTGACGAGATCGGAAGAGCA45460.10274827914713955No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)