FastQCFastQC Report
Fri 14 Jul 2023
SRR9691663_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691663_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3663135
Total Bases278.3 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA892622.436765229782686No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA783252.1381958349883363Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC741392.0239221322719474No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG738152.0150772494052225No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA301960.8243212439617977No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA260300.7105935216692805Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG240420.6563230675364135No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA232950.6359306987047979Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCACAC221430.6044822262897763No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG217360.5933715246639831No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA205610.5612951747615089No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA188790.515378221113882Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAA169920.463864968121568No Hit
GATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCA145920.3983473172569397No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC139820.3816949143288467Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATATCATTCTGACCTGTAGCGTTCGAGATCGGAAGAGCACACGTCTGAAC139810.38166761530765314Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA139380.38049375739632857No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT138890.3791561053578424Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT137730.37598941889938536No Hit
TGTGGGCACTCGAAGATACGGATTCTTAAGATCGGAAGAGCACACGTCTG106630.2910894629873046Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC105860.2889874383553978No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG91070.2486121860100706No Hit
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC90630.2474110290775524No Hit
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA79840.21795538520966332Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCA76080.20769095324087156No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA75190.20526134035464158No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAGATCGGA67540.18437758914154131No Hit
GGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAG63940.1745499415118471No Hit
ATGGCGTCGGCAAGACCCGTTCTAAAGCCATCCTGGCTGCAGCGGGTATC62400.17034589224803345No Hit
TCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACA61830.16878984803999852No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA59620.162756764356214Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGA59110.16136451427534065No Hit
ATATCATTCTGACCTGTAGCGTTCGTAAGATCGGAAGAGCACACGTCTGA56870.15524953352797535Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATATCATTCTGACCTGTAGCGTTCGTACCCAGATCGGAAGAGCACACGTC55700.15205554804832472No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG55510.15153686664564642No Hit
ATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGA54370.1484247782295766No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA50960.13911581200256065No Hit
AGGACGGGGATCAAGAGAGGTCAGATCGGAAGAGCACACGTCTGAACTCC49600.13540314512023172Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACAACGTCGTCGTACTGGGCACCCAATGGGGTGACGAGATCGGAAGAGCA49010.1337925028698096No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC48650.13280973810684019Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG47910.13078961053851415No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA47040.12841459569467137No Hit
ATATCATTCTGACCTGTAGCGTTCGTACCCAGAGATCGGAAGAGCACACG46320.12644906616873253No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGAGATCGGAAGAGCA46030.12565739455411826No Hit
TTCTGGCTGAACAACTGGAAGCTTTCGTTGACTTACGAGATCGGAAGAGC45430.12401945328250256No Hit
AACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGG43110.1176860803655885No Hit
TCATTCTGACCTGTAGCGTTCGTACCCAGATCGGAAGAGCACACGTCTGA43090.11763148232320131Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGGA42290.11544756062771369No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACATCGATCTCGTATG41330.11282685459312856TruSeq Adapter, Index 8 (97% over 37bp)
AGTTATGCGTCTGGATCGTCTTACTAGATCGGAAGAGCACACGTCTGAAC41210.11249926633880541Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCA40490.11053373681286656No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC39620.10815872196902379Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTACTGTAGAGTCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAG39600.10810412392663661Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGA39380.10750354546037752No Hit
ATGAAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACA39250.10714865818486079No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA38390.1048009423622116No Hit
AGTTATGCGTCTGGATCGTCTAGATCGGAAGAGCACACGTCTGAACTCCA37890.10343599130253184Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGACGGGGATCAAGAGAGGTAGATCGGAAGAGCACACGTCTGAACTCCA37490.10234403045478804Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAGATCGGAA36870.10065149114078516No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)