FastQCFastQC Report
Fri 14 Jul 2023
SRR9691664_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691664_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3630119
Total Bases275.8 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA880472.425457677833702No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA773092.129654702779716Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC738252.0336798876290283No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG736382.0285285413508483No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA295960.8152900772674394No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA255970.7051283993720316Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG232800.641301290673942No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA226310.623423088884965Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCACAC220010.6060682859156958No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG212420.5851598804336717No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA202960.5591001286734678No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA188780.5200380483394621Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAA164540.4532633778672269No Hit
GATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCA139700.3848358690169661No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC139060.38307284141373876Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA138600.38180566532391913No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT136400.37574525793782515Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATATCATTCTGACCTGTAGCGTTCGAGATCGGAAGAGCACACGTCTGAAC135560.37343128420858934Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT134160.3695746613265295No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC103550.28525235674092225No Hit
TGTGGGCACTCGAAGATACGGATTCTTAAGATCGGAAGAGCACACGTCTG101820.28048667275094835Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC90620.24963368969447008No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG87730.24167251817364663No Hit
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA80560.22192109955624043Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCA75400.20770668950522006No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA73610.20277572167744362No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAGATCGGA66420.18296920844743658No Hit
ATGGCGTCGGCAAGACCCGTTCTAAAGCCATCCTGGCTGCAGCGGGTATC62490.17214311707136873No Hit
GGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAG61820.17029744754924012No Hit
TCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACA61590.1696638595043303No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA60720.16726724385619313Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGA58400.16087626879449407No Hit
ATATCATTCTGACCTGTAGCGTTCGTAAGATCGGAAGAGCACACGTCTGA57180.15751549742584198Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATATCATTCTGACCTGTAGCGTTCGTACCCAGATCGGAAGAGCACACGTC55300.15233660384136166No Hit
ATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGA54580.15035319778773093No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG53670.14784639291439206No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA50410.13886597106045284No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC49260.13569803083590373Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGACGGGGATCAAGAGAGGTCAGATCGGAAGAGCACACGTCTGAACTCC48670.13407273976417852Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG46740.12875610964819612No Hit
ACAACGTCGTCGTACTGGGCACCCAATGGGGTGACGAGATCGGAAGAGCA46470.12801233237808457No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA46390.12779195392768117No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGAGATCGGAAGAGCA45990.12669006167566407No Hit
ATATCATTCTGACCTGTAGCGTTCGTACCCAGAGATCGGAAGAGCACACG45560.12550552750474572No Hit
TTCTGGCTGAACAACTGGAAGCTTTCGTTGACTTACGAGATCGGAAGAGC44550.12272324956840257No Hit
AACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGG42340.11663529487600821No Hit
TCATTCTGACCTGTAGCGTTCGTACCCAGATCGGAAGAGCACACGTCTGA41530.11440396306567359Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGGA40670.11203489472383689No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGA40550.11170432704823176No Hit
AGTTATGCGTCTGGATCGTCTTACTAGATCGGAAGAGCACACGTCTGAAC40010.1102167725080087Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACATCGATCTCGTATG39600.10908733294969118TruSeq Adapter, Index 8 (97% over 37bp)
TGTACTGTAGAGTCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAG39550.10894959641818903Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCA39170.10790279877877282No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA38180.10517561545503053No Hit
ATGAAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACA38160.10512052084242969No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC38000.10467976394162284Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGACGGGGATCAAGAGAGGTAGATCGGAAGAGCACACGTCTGAACTCCA37760.1040186285904126Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTTATGCGTCTGGATCGTCTAGATCGGAAGAGCACACGTCTGAACTCCA36640.10093333028476477Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTGGGCACTCGAAGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC36510.10057521530285922Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph