FastQCFastQC Report
Fri 14 Jul 2023
SRR9691665_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691665_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3487521
Total Bases265 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA863652.476400858948233No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA768472.2034849395888942Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC707462.028546924878732No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG702702.0148982615445186No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA289890.8312208012510893No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA244450.7009276790017895Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA228670.6556806396291234Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG225020.6452147528287284No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCACAC207890.5960967690230395No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG203330.5830215789381626No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA189230.5425917148599249No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA180840.51853451205025Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAA162790.46677855129761225No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC138300.39655675191633255Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATATCATTCTGACCTGTAGCGTTCGAGATCGGAAGAGCACACGTCTGAAC134290.3850586132671316Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT132660.3803848062850374Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA131890.3781769342750911No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT130380.37384721124259895No Hit
GATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCA129550.37146729725785166No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC102660.29436381888453145No Hit
TGTGGGCACTCGAAGATACGGATTCTTAAGATCGGAAGAGCACACGTCTG101450.29089430572604436Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC88920.2549662066550997No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG88710.2543640597432962No Hit
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA77730.22288037835471097Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCA73600.21103815575590798No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA71660.20547546523734195No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAGATCGGA64170.18399889204968228No Hit
ATGGCGTCGGCAAGACCCGTTCTAAAGCCATCCTGGCTGCAGCGGGTATC61370.17597026655896839No Hit
TCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACA59610.17092370196480539No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA58320.16722479950658362Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAG57750.165590400745974No Hit
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGA57380.1645294752347011No Hit
ATATCATTCTGACCTGTAGCGTTCGTAAGATCGGAAGAGCACACGTCTGA55780.15994168924000743Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATATCATTCTGACCTGTAGCGTTCGTACCCAGATCGGAAGAGCACACGTC54440.15609941846945152No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG53320.15288796827316595No Hit
ATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGA51170.14672313084279637No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA49790.14276616542237308No Hit
AGGACGGGGATCAAGAGAGGTCAGATCGGAAGAGCACACGTCTGAACTCC48750.1397841045258222Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC47360.13579846544293211Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACAACGTCGTCGTACTGGGCACCCAATGGGGTGACGAGATCGGAAGAGCA46670.13381998273272044No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG45320.1299490382996977No Hit
ATATCATTCTGACCTGTAGCGTTCGTACCCAGAGATCGGAAGAGCACACG44140.12656554612861112No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA43910.12590605189187393No Hit
TTCTGGCTGAACAACTGGAAGCTTTCGTTGACTTACGAGATCGGAAGAGC43570.12493114736800151No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGAGATCGGAAGAGCA43560.12490247370553467No Hit
TCATTCTGACCTGTAGCGTTCGTACCCAGATCGGAAGAGCACACGTCTGA42480.12180571815911646Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGG40090.11495271282954281No Hit
AGTTATGCGTCTGGATCGTCTTACTAGATCGGAAGAGCACACGTCTGAAC39080.11205667292039245Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGGA38500.11039360049731599No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGA37690.10807103383750234No Hit
ACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCA37570.10772694988790033No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACATCGATCTCGTATG37230.10675204536402792TruSeq Adapter, Index 8 (97% over 37bp)
ATGAAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACA36830.1056050988653545No Hit
TGTACTGTAGAGTCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAG36550.10480223631628312Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGACGGGGATCAAGAGAGGTAGATCGGAAGAGCACACGTCTGAACTCCA36270.10399937376721173Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTTATGCGTCTGGATCGTCTAGATCGGAAGAGCACACGTCTGAACTCCA35910.10296712191840565Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC35880.10288110093100515Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT35270.10113200752052819Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)