FastQCFastQC Report
Fri 14 Jul 2023
SRR9691666_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691666_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3578419
Total Bases271.9 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA881472.463294544322507No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA780322.180627813567947Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC724762.0253637150931736No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG719332.0101894160521727No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA301830.8434730533232694No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA254130.7101739623001108Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG232870.6507622500327659No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA231280.6463189469986605Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCACAC216270.6043730485446226No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG209740.5861247662724796No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA195610.5466380544033552No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA185920.5195590566672041Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAA165000.4610974846713032No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC139810.39070326867815086Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA138880.3881043555827308No Hit
ATATCATTCTGACCTGTAGCGTTCGAGATCGGAAGAGCACACGTCTGAAC137070.38304625590239716Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCA135640.37905007770191246No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT135170.3777366485031518Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT133350.3726506035207168No Hit
TGTGGGCACTCGAAGATACGGATTCTTAAGATCGGAAGAGCACACGTCTG105380.2944875935434056Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC104300.2914695009164662No Hit
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC91580.2559230766436239No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG89070.2489088058161998No Hit
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA79220.2213826832464281Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA74770.20894702381135358No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCA73260.2047272831940586No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAGATCGGA66460.18572447776518064No Hit
GGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAG62300.17409923209104355No Hit
ATGGCGTCGGCAAGACCCGTTCTAAAGCCATCCTGGCTGCAGCGGGTATC61110.17077374114098992No Hit
TCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACA59920.1674482501909363No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA58910.16462577467870587Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGA57690.16121644782234834No Hit
ATATCATTCTGACCTGTAGCGTTCGTAAGATCGGAAGAGCACACGTCTGA57130.15965151090467605Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATATCATTCTGACCTGTAGCGTTCGTACCCAGATCGGAAGAGCACACGTC55990.15646574646512887No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG55670.15557149679788757No Hit
ATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGA53870.15054134241965514No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA50990.14249309541448332No Hit
AGGACGGGGATCAAGAGAGGTCAGATCGGAAGAGCACACGTCTGAACTCC50600.14140322863253296Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC49330.137854175265669Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACAACGTCGTCGTACTGGGCACCCAATGGGGTGACGAGATCGGAAGAGCA47350.13232100544961337No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA45930.12835277255123No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG45360.12675989033145643No Hit
ATATCATTCTGACCTGTAGCGTTCGTACCCAGAGATCGGAAGAGCACACG44970.1256700235495061No Hit
TTCTGGCTGAACAACTGGAAGCTTTCGTTGACTTACGAGATCGGAAGAGC44570.12455221146545443No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGAGATCGGAAGAGCA44480.12430070374654281No Hit
TCATTCTGACCTGTAGCGTTCGTACCCAGATCGGAAGAGCACACGTCTGA42360.11837629970106911Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGG40970.11449190270898964No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACATCGATCTCGTATG39930.11158559129045537TruSeq Adapter, Index 8 (97% over 37bp)
AGTTATGCGTCTGGATCGTCTTACTAGATCGGAAGAGCACACGTCTGAAC39540.110495724508505Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGGA39430.1101883261853908No Hit
ACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCA39370.11002065437278306No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGA39140.10937791242445337No Hit
AGGACGGGGATCAAGAGAGGTAGATCGGAAGAGCACACGTCTGAACTCCA37860.10580091375548811Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTACTGTAGAGTCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAG37540.1049066640882468Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATGAAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACA36630.1023636415970293No Hit
AGTTATGCGTCTGGATCGTCTAGATCGGAAGAGCACACGTCTGAACTCCA36320.10149733723188926Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA36250.10130172011718025No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC35870.10023979863733118Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)