FastQCFastQC Report
Fri 14 Jul 2023
SRR9691667_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691667_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5588507
Total Bases424.7 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA1731683.098645130085728Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC699261.2512465314975896No Hit
TCGACACACTATCATTAACTGAATCAGATCGGAAGAGCACACGTCTGAAC653731.169775755850355Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG545460.9760388597527031Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG517380.9257928817124145No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG475800.8513901834604484No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC463870.8300428003400551No Hit
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA348820.6241738625360942No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA325800.5829821811084785No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA317490.5681123777781794No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAA314560.5628694747989043No Hit
AAGAATGTGGCGCTCCGCGGTTAGATCGGAAGAGCACACGTCTGAACTCC250420.44809821299320196Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA247870.4435352769532185No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA223120.3992479565651434Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA221250.3959018034691555No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTTAGATCGGAAGAG219190.39221566690352183No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGA217060.38840427327012383No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC208590.37324816807064926No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTTAGATCGGAAGA195370.34959247613002903No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC163410.29240367776223597No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC159240.2849419352968512No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC153620.27488558214206404Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGAATGTGGCGCTCCGCGGTTGAGATCGGAAGAGCACACGTCTGAACTC152260.27245201625407284Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGAATGTGGCGCTCCGCGGTTAGATCGGAAGAGCACACGTCTGAACTCCA150530.2693563772936135Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTTAGATCGGAAGAGCAC138580.24797320643957324No Hit
TCGACACACTATCATTAACTGAATAGATCGGAAGAGCACACGTCTGAACT134890.24137036958171476Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGGACGTGGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC134350.24040410077324764Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG133950.2396883461003091No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA129570.23185083243163154Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC128390.22973935614646274No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTGTATCTCGTATG125860.22521220784012616TruSeq Adapter, Index 7 (97% over 35bp)
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC123710.22136502647308126Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCGTTAGCGGTAGCTGTCCGCTGTATCTGGATGAGATCGGAAGAGCACA113650.20336379644867583No Hit
TTCGACACACTATCATTAACTGAATCAGATCGGAAGAGCACACGTCTGAA111820.2000892188199818Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT111620.19973134148351251Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT110270.19731566946234474Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC109890.1966357025230531Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTTAGATCGGAAG107890.1930569291583602No Hit
AGCGGTAGCTGTCCGCTGTATCTGGATGAGATCGGAAGAGCACACGTCTG106960.19139279954377797Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCATCATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTG106860.19121386087554332Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCGTTGTCTGACACCGGAAGATGAGATCGGAAGAGCACACGTCTGAACT102830.18400263254568708Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA99980.17890288050099964No Hit
AGAATGTGGCGCTCCGCGGTTGAGATCGGAAGAGCACACGTCTGAACTCC99100.17732822022053474Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG98480.17621880047747995No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGA97030.17362418978807756Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCTGGGTACCGAGAACCTGTACTTCCAGATCGGAAGAGCACACGTCTGA94950.16990226548879692Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTGGGTACCGAGAACCTGTACTTCCAGATCGGAAGAGCACACGTCTGAA90550.16202896408647247Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTGGGCACTCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC85310.152652577870977Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGTTGTCTGACACCGGAAGATGCAGATCGGAAGAGCACACGTCTGAACT83070.1486443517025209Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGAGATCGGA79890.14295410205265913No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC79160.14164784977454623No Hit
ATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTGAACTCC78260.1400374017604344Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA78210.1399479324263171TruSeq Adapter, Index 7 (100% over 35bp)
AGCGGTAGCTGTCCGCTGTATCTGGATGAAATTCGAGATCGGAAGAGCAC77910.13941111642161313No Hit
TTTCGACACACTATCATTAACTGAATCAGATCGGAAGAGCACACGTCTGA76790.1374070033373851Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGA74380.1330945814329301No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTTAGATCGGAAGAGCA72510.12974842833694222No Hit
TTAGCGGTAGCTGTCCGCTGTATCTGGATGAGATCGGAAGAGCACACGTC71720.12833481285788853No Hit
AAGAATGTGGCGCTCCGCGGTAGATCGGAAGAGCACACGTCTGAACTCCA71090.12720749924801025Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACT67320.12046151145556407Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTC64890.11611330181746216No Hit
TGGCCAGGCTGTCTCCACCCGAGAGATCGGAAGAGCACACGTCTGAACTC64410.11525439620993586Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA64160.11480704953934924Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGAGATCGG63870.11428812740146875No Hit
CGGACGTGGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC58780.10518014918832524Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC58460.10460754544997439Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCGGTAGCTGTCCGCTGTATCTGGATGAAATTCGTCAGATCGGAAGAGC57650.10315814223727374No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)