FastQCFastQC Report
Fri 14 Jul 2023
SRR9691669_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691669_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5314076
Total Bases403.8 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA1644103.093858650120924Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC658371.2389171701721993No Hit
TCGACACACTATCATTAACTGAATCAGATCGGAAGAGCACACGTCTGAAC618101.1631372979987489Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG522570.9833694512460868Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG485230.9131032375148568No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG451090.848858766792195No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC430130.8094163500860733No Hit
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA322750.6073492362548071No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA312690.5884183816716207No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAA304350.57272421395554No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA302320.5689041707344795No Hit
AAGAATGTGGCGCTCCGCGGTTAGATCGGAAGAGCACACGTCTGAACTCC242790.4568809328282094Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA237330.44660633381984No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA210410.3959484207602601Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGA209440.3941230799107878No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTTAGATCGGAAGAG206220.38806370100841614No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA205910.3874803446544611No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC196190.3691893002659352No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTTAGATCGGAAGA182680.3437662540016364No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTGTATCTCGTATG169360.3187007487284713TruSeq Adapter, Index 7 (97% over 35bp)
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC153180.2882533106413984No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC151180.2844897212610433No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC145190.2732177710668797Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGAATGTGGCGCTCCGCGGTTGAGATCGGAAGAGCACACGTCTGAACTC144280.27150533789881814Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGAATGTGGCGCTCCGCGGTTAGATCGGAAGAGCACACGTCTGAACTCCA141770.2667820332264725Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTTAGATCGGAAGAGCAC127580.24007936657285292No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG126910.23881856413043395No Hit
TCGACACACTATCATTAACTGAATAGATCGGAAGAGCACACGTCTGAACT126720.2384610231393002Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGGACGTGGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC125930.23697440533405997Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA125810.23674858997123865Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC121880.22935313683884084No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC119580.22502500905143244Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCGTTAGCGGTAGCTGTCCGCTGTATCTGGATGAGATCGGAAGAGCACA107220.20176602668083785No Hit
TTCGACACACTATCATTAACTGAATCAGATCGGAAGAGCACACGTCTGAA106680.20074985754814195Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC103340.1944646632829489Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT103270.19433293765463647Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT102460.19280868395559267Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGGTAGCTGTCCGCTGTATCTGGATGAGATCGGAAGAGCACACGTCTG102160.19224414554853939Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCATCATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTG101400.19081398158400445Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTTAGATCGGAAG99890.18797247160183633No Hit
TTCGTTGTCTGACACCGGAAGATGAGATCGGAAGAGCACACGTCTGAACT98040.18449115142500785Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAATGTGGCGCTCCGCGGTTGAGATCGGAAGAGCACACGTCTGAACTCC93870.17664406756696743Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGA93150.17528917539003958Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA91160.17154440395658624No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG89930.16922979648766784No Hit
ATCTGGGTACCGAGAACCTGTACTTCCAGATCGGAAGAGCACACGTCTGA89730.16885343754963233Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTGGGTACCGAGAACCTGTACTTCCAGATCGGAAGAGCACACGTCTGAA87190.16407367903658135Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTGGGCACTCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC83460.15705458484221904Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGTTGTCTGACACCGGAAGATGCAGATCGGAAGAGCACACGTCTGAACT76780.14448419631183296Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGAGATCGGA76210.14341157333843174No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC75440.14196259142699502No Hit
AGCGGTAGCTGTCCGCTGTATCTGGATGAAATTCGAGATCGGAAGAGCAC75080.1412851453385311No Hit
ATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTGAACTCC74130.1394974403828624Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA73770.1388199942943985TruSeq Adapter, Index 7 (100% over 35bp)
TTTCGACACACTATCATTAACTGAATCAGATCGGAAGAGCACACGTCTGA71840.13518813054235582Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGA70900.1334192435335889No Hit
AAGAATGTGGCGCTCCGCGGTAGATCGGAAGAGCACACGTCTGAACTCCA68890.129636836206332Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTTAGATCGGAAGAGCA67540.1270964133745923No Hit
TTAGCGGTAGCTGTCCGCTGTATCTGGATGAGATCGGAAGAGCACACGTC67420.126870598011771No Hit
ATTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACT63890.12022786275544423Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTC62010.1166900887379104No Hit
AAAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGAGATCGG61770.1162384580122678No Hit
TGGCCAGGCTGTCTCCACCCGAGAGATCGGAAGAGCACACGTCTGAACTC60500.1138485787557423Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA60020.11294531730445707Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAAC55970.10532404880923797Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGACGTGGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC55540.10451487709246161Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCGGTAGCTGTCCGCTGTATCTGGATGAAATTCGTCAGATCGGAAGAGC54570.10268953624298938No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC53330.10035611082716919Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)