FastQCFastQC Report
Fri 14 Jul 2023
SRR9691670_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691670_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5087094
Total Bases386.6 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA1632453.2090030182261224Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC639931.2579480544295034No Hit
TCGACACACTATCATTAACTGAATCAGATCGGAAGAGCACACGTCTGAAC600851.1811261989654604Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG517341.0169656782438068Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG469800.9235135029940472No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG437600.8602160683486485No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC416550.8188368447683491No Hit
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA307850.6051588588691305No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA294950.5798005698341725No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA293230.5764194646295115No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAA287660.5654701878911614No Hit
AAGAATGTGGCGCTCCGCGGTTAGATCGGAAGAGCACACGTCTGAACTCC233830.4596533895383101Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA227810.4478195213219964No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTGTATCTCGTATG224950.44219745103982744TruSeq Adapter, Index 7 (97% over 35bp)
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA210260.4133204536814141Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA199650.39246375238987136No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGA197520.38827668606084337No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTTAGATCGGAAGAG196530.38633058480932336No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC189780.37306171263986865No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTTAGATCGGAAGA180440.35470152507502317No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC148710.2923279970843865No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC145680.28637174779943125No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC140880.27693610536781904Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGAATGTGGCGCTCCGCGGTTGAGATCGGAAGAGCACACGTCTGAACTC139370.27396780951954103Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGAATGTGGCGCTCCGCGGTTAGATCGGAAGAGCACACGTCTGAACTCCA137520.27033115566569044Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGACACACTATCATTAACTGAATAGATCGGAAGAGCACACGTCTGAACT124800.24532670322191807Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGGACGTGGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC124700.24513012733792613Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTTAGATCGGAAGAGCAC124470.24467800280474472No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA123520.24281053190682145Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG121210.23826962898660808No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC117360.23070145745291906Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC115670.22737932501345565No Hit
TTCGACACACTATCATTAACTGAATCAGATCGGAAGAGCACACGTCTGAA100210.19698869334830454Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCGTTAGCGGTAGCTGTCCGCTGTATCTGGATGAGATCGGAAGAGCACA100100.19677245987591344No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT99530.1956519773371595Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGGTAGCTGTCCGCTGTATCTGGATGAGATCGGAAGAGCACACGTCTG98660.19394176714642977Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT98640.19390245196963138Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTTAGATCGGAAG98290.19321443637565966No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC97690.19203498107170813Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCATCATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTG96910.19050168917657115Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCGTTGTCTGACACCGGAAGATGAGATCGGAAGAGCACACGTCTGAACT94890.18653085631993432Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGA91380.17963104279181788Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA90170.17725247459551563No Hit
AGAATGTGGCGCTCCGCGGTTGAGATCGGAAGAGCACACGTCTGAACTCC89680.1762892527639552Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG89020.17499185192960853No Hit
ATCTGGGTACCGAGAACCTGTACTTCCAGATCGGAAGAGCACACGTCTGA88860.17467733051522147Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTGGGTACCGAGAACCTGTACTTCCAGATCGGAAGAGCACACGTCTGAA83360.16386565689566576Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTGGGCACTCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC80340.15792906519910976Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGTTGTCTGACACCGGAAGATGCAGATCGGAAGAGCACACGTCTGAACT75620.14865068347469104Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTGAACTCC73190.14387388949368737Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCGGTAGCTGTCCGCTGTATCTGGATGAAATTCGAGATCGGAAGAGCAC72980.14346108013730433No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA72830.14316621631131646TruSeq Adapter, Index 7 (100% over 35bp)
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGAGATCGGA72340.14220299447975604No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC72330.14218333689135682No Hit
TTTCGACACACTATCATTAACTGAATCAGATCGGAAGAGCACACGTCTGA68840.13532283854003876Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGA67430.13255111857575266No Hit
TTAGCGGTAGCTGTCCGCTGTATCTGGATGAGATCGGAAGAGCACACGTC66250.130231523144648No Hit
AAGAATGTGGCGCTCCGCGGTAGATCGGAAGAGCACACGTCTGAACTCCA65460.12867857366111182Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTTAGATCGGAAGAGCA64590.12696836347038212No Hit
ATTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACT61550.1209924565970277Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA59230.11643189608841513Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTC58950.11588148361323773No Hit
TGGCCAGGCTGTCTCCACCCGAGAGATCGGAAGAGCACACGTCTGAACTC58610.11521312560766521Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGAGATCGG57600.1132277091793468No Hit
TTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAAC53840.10583645594125055Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGACGTGGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC53400.1049715220516861Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCGGTAGCTGTCCGCTGTATCTGGATGAAATTCGTCAGATCGGAAGAGC52110.1024356931481903No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC52110.1024356931481903Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)