FastQCFastQC Report
Fri 14 Jul 2023
SRR9691671_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691671_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5199833
Total Bases395.1 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG945941.8191738080819135No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC818011.5731466760567119No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA714441.3739672024082312No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG676221.3004648418516518No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA528881.0171095879425358Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC495760.9534152346815754No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA419830.8073913142979785No Hit
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA361830.6958492705438809No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA305770.5880381158394895No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA291790.5611526370173812No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG267370.5141895903195353No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGGCATCTCGTATG247990.47691916259618333TruSeq Adapter, Index 12 (97% over 37bp)
AGAATGTGGCGCTCCGCGGTTAGATCGGAAGAGCACACGTCTGAACTCCA204250.39280107649611057Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCATCATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTG190870.3670694808852515Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC184220.3542806086272386No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC184170.35418445169296786No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT179790.34576110425084805No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG171470.32976059038819133No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC155600.29924037945064774No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG144870.2786051013561397Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCGGTAGCTGTCCGCTGTATCTGGATGAAATTCGAGATCGGAAGAGCAC135460.2605083663263801No Hit
AGCGGTAGCTGTCCGCTGTATCTGGATGAGATCGGAAGAGCACACGTCTG134020.2577390466193818Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG131390.2526811918767391No Hit
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT128710.24752718019982567Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCGTTAGCGGTAGCTGTCCGCTGTATCTGGATGAGATCGGAAGAGCACA123320.23716146268543625No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC120080.23093049334469012No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA115210.22156480794671674Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG114450.22010322254580098No Hit
AGAATGTGGCGCTCCGCGGTTGAGATCGGAAGAGCACACGTCTGAACTCC102760.1976217313132941Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC94750.18221739044311616No Hit
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA90140.17335172110335081Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCATCATTCTTCTGGTGTAGATCTGGAGATCGGAAGAGCACACGTCTGA88240.1696977576010614Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA85730.16487067950066858No Hit
ATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTGAACTCC84150.16183212037771214Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCGGTAGCTGTCCGCTGTATCTGGATGAAATTCGTCAGATCGGAAGAGC82200.15808199994115196No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT82200.15808199994115196TruSeq Adapter, Index 1 (100% over 35bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC82120.1579281488463187Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA77260.1485816948351995Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTAGCGGTAGCTGTCCGCTGTATCTGGATGAAATTCGAGATCGGAAGAGC74440.1431584437423279No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA74230.14275458461839063No Hit
TTAGCGGTAGCTGTCCGCTGTATCTGGATGAAATTCGTCAGATCGGAAGA72530.13948524885318433No Hit
AATCCTGCTCTGCGAGGCTGGCCGTAGGCCGGCCCCGAGATCGGAAGAGC72530.13948524885318433No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA71410.137331333525519No Hit
AAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGAGATCGG69780.13419661746829176No Hit
AGCGAATTTCGTTGTCTGACACCGGAGATCGGAAGAGCACACGTCTGAAC69380.13342736199412558Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA65340.12565788170504708Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACT63960.1230039503191737Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTATGAGCGTTAGCGGTAGCTGTCCGAGATCGGAAGAGCACACGTCTGA63500.1221193065238826Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT62280.11977307732767573Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTAGCGGTAGCTGTCCGCTGTATCTGGATGAGATCGGAAGAGCACACGTC60280.11592679995684477No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC60230.11583064302257401No Hit
TTCGTTGTCTGACACCGGAAGATGAGATCGGAAGAGCACACGTCTGAACT59760.11492676784042873Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG59540.11450367732963732No Hit
TGTCCGGAACTGAGCCTGGGTTGGTTTCAGATCGGAAGAGCACACGTCTG58370.11225360506770123Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGACGTGGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC56840.10931120287901554Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG56620.10888811236822414No Hit
AGCTGTCCGCTGTATCTGGATGAAATTCGTCAGATCGGAAGAGCACACGT54560.10492644667626826No Hit
ATTCGTCATTTTCTGACCCTGTGTCCGGAGATCGGAAGAGCACACGTCTG54440.1046956700340184Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGAGATCGGA53910.1036764065307482No Hit
TTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACTC53470.1028302255091654Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA53130.10217635835612414No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA52970.10186865616645766Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC52340.1006570787946459Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)