FastQCFastQC Report
Fri 14 Jul 2023
SRR9691673_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691673_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5470053
Total Bases415.7 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG989761.8094157405787477No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC865061.5814471998717379No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA751821.3744290960252121No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG714831.3068063508708234No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA558471.020959029098987Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC526800.9630619666756428No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA441190.8065552564115924No Hit
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA386690.7069218524939338No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA324010.5923342973093679No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA304060.5558629870679498No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG281950.5154429033868593No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGGCATCTCGTATG251660.46006866843886157TruSeq Adapter, Index 12 (97% over 37bp)
AGAATGTGGCGCTCCGCGGTTAGATCGGAAGAGCACACGTCTGAACTCCA209730.38341493217707395Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCATCATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTG199600.3648959159993514Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC195000.35648649108153063No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC188840.34522517423505766No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT188640.34485954706471766No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG182300.33326916576493865No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC159400.291404854761005No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG150790.2756646050778667Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCGGTAGCTGTCCGCTGTATCTGGATGAAATTCGAGATCGGAAGAGCAC148380.27125879767526934No Hit
AGCGGTAGCTGTCCGCTGTATCTGGATGAGATCGGAAGAGCACACGTCTG139740.25546370391657997Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG138610.25339791040415877No Hit
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT134530.24593911612922215Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCGTTAGCGGTAGCTGTCCGCTGTATCTGGATGAGATCGGAAGAGCACA131170.23979657966750964No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC126900.23199043958074994No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA122470.22389179775771823Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG121140.22146037707495703No Hit
AGAATGTGGCGCTCCGCGGTTGAGATCGGAAGAGCACACGTCTGAACTCC108250.19789570594654204Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC98030.1792121575421664No Hit
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA95890.17529994681952807Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCATCATTCTTCTGGTGTAGATCTGGAGATCGGAAGAGCACACGTCTGA93780.17144258017244074Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA92240.16862725096082248No Hit
ATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTGAACTCC88510.1618083042339809Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT88090.16104048717626684TruSeq Adapter, Index 1 (100% over 35bp)
AGCGGTAGCTGTCCGCTGTATCTGGATGAAATTCGTCAGATCGGAAGAGC87390.15976079208007674No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC84230.15398388278870423Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA82440.15071151961416096Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA78920.1442764814161764No Hit
TTAGCGGTAGCTGTCCGCTGTATCTGGATGAAATTCGAGATCGGAAGAGC76580.13999864352319805No Hit
TTAGCGGTAGCTGTCCGCTGTATCTGGATGAAATTCGTCAGATCGGAAGA75710.1384081653322189No Hit
AATCCTGCTCTGCGAGGCTGGCCGTAGGCCGGCCCCGAGATCGGAAGAGC75090.1372747211041648No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA73940.13517236487470963No Hit
AAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGAGATCGG72110.13182687626609835No Hit
AGCGAATTTCGTTGTCTGACACCGGAGATCGGAAGAGCACACGTCTGAAC71450.13062030660397622Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACT70430.12875560803524208Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA69830.12765872652422197Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTATGAGCGTTAGCGGTAGCTGTCCGAGATCGGAAGAGCACACGTCTGA67210.12286901059276756Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT66870.1222474444031895Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTAGCGGTAGCTGTCCGCTGTATCTGGATGAGATCGGAAGAGCACACGTC64160.1172931962450821No Hit
TTCGTTGTCTGACACCGGAAGATGAGATCGGAAGAGCACACGTCTGAACT62280.11385630084388579Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG61880.11312504650320572No Hit
TGTCCGGAACTGAGCCTGGGTTGGTTTCAGATCGGAAGAGCACACGTCTG61380.11221097857735565Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC61310.11208300906773663No Hit
ACGGACGTGGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC58770.10743954400441824Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCTGTCCGCTGTATCTGGATGAAATTCGTCAGATCGGAAGAGCACACGT58290.10656203879560217No Hit
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG58070.10615984890822813No Hit
TTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACTC57100.10438655713207896Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTCGTCATTTTCTGACCCTGTGTCCGGAGATCGGAAGAGCACACGTCTG57000.10420374354690896Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGAGATCGGA55920.10222935682707279No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC55080.10069372271164466Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA54940.10043778369240663No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)