FastQCFastQC Report
Fri 14 Jul 2023
SRR9691674_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691674_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5425681
Total Bases412.3 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG972201.79184880202135No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC842971.55366671944038No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA726521.3390392837323093No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG697871.2862348523623117No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA531870.9802824751399871Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC509930.9398451549215665No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA438560.8083040635820646No Hit
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA370630.6831031901801821No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA327150.6029657843872502No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA302270.5571097895360969No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG278240.5128204183032508No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGGCATCTCGTATG242030.446082252163369TruSeq Adapter, Index 12 (97% over 37bp)
AGAATGTGGCGCTCCGCGGTTAGATCGGAAGAGCACACGTCTGAACTCCA210830.38857794993845013Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCATCATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTG196380.3619453484272297Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC190030.3502417484551709No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC189790.34979940766882534No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT185440.3417819809163126No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG175180.32287191230004125No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC159480.2939354525266045No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG146340.26971729447418674Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCGGTAGCTGTCCGCTGTATCTGGATGAAATTCGAGATCGGAAGAGCAC143590.26464880629731086No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG140550.25904582300360085No Hit
AGCGGTAGCTGTCCGCTGTATCTGGATGAGATCGGAAGAGCACACGTCTG137110.2527056050659816Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT135650.25001469861571296Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCGTTAGCGGTAGCTGTCCGCTGTATCTGGATGAGATCGGAAGAGCACA132530.24426426839322105No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC125360.23104933740114836No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA118370.21816616199883482Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG117200.21600975066540037No Hit
AGAATGTGGCGCTCCGCGGTTGAGATCGGAAGAGCACACGTCTGAACTCC107480.1980949488184064Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC99120.18268674476070376No Hit
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA94490.17415325375745458Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCATCATTCTTCTGGTGTAGATCTGGAGATCGGAAGAGCACACGTCTGA90430.16667032212177604Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA89060.16414529346638698No Hit
ATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTGAACTCC88830.1637213835461392Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCGGTAGCTGTCCGCTGTATCTGGATGAAATTCGTCAGATCGGAAGAGC87160.16064342890781821No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT85110.15686510135778348TruSeq Adapter, Index 1 (100% over 35bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC83950.15472712089044674Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA81520.15024842042869826Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA77280.14243373320326058No Hit
TTAGCGGTAGCTGTCCGCTGTATCTGGATGAAATTCGAGATCGGAAGAGC76450.14090397131714893No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA75730.13957694895811235No Hit
TTAGCGGTAGCTGTCCGCTGTATCTGGATGAAATTCGTCAGATCGGAAGA74800.13786287841102343No Hit
AAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGAGATCGG74190.13673859557906187No Hit
AATCCTGCTCTGCGAGGCTGGCCGTAGGCCGGCCCCGAGATCGGAAGAGC74060.13649899431979137No Hit
AGCGAATTTCGTTGTCTGACACCGGAGATCGGAAGAGCACACGTCTGAAC70040.12908978614850378Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACT67060.1235973880513801Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTATGAGCGTTAGCGGTAGCTGTCCGAGATCGGAAGAGCACACGTCTGA66600.1227495682108845Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA65350.12044570994866821Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT65270.12029826301988636Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTAGCGGTAGCTGTCCGCTGTATCTGGATGAGATCGGAAGAGCACACGTC63630.11727560097985856No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC61730.1137737364212898No Hit
TGTCCGGAACTGAGCCTGGGTTGGTTTCAGATCGGAAGAGCACACGTCTG61700.1137184438229966Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCGTTGTCTGACACCGGAAGATGAGATCGGAAGAGCACACGTCTGAACT61040.1125020066605464Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG59840.11029030272881875No Hit
ACGGACGTGGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC58750.10828133832416613Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG58290.10743351848367053No Hit
TTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACTC57260.10553513927560429Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCTGTCCGCTGTATCTGGATGAAATTCGTCAGATCGGAAGAGCACACGT57140.10531396888243154No Hit
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGAGATCGGA56130.10345245140656076No Hit
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA55530.10234659944069693No Hit
ATTCGTCATTTTCTGACCCTGTGTCCGGAGATCGGAAGAGCACACGTCTG54360.10019018810726248Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)