FastQCFastQC Report
Fri 14 Jul 2023
SRR9691675_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691675_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5618552
Total Bases427 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG1756913.1269800475282596No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA1116891.9878609292928142No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA779161.3867629951631666No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA627771.1173163476995496No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA413320.7356343769711484Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC402100.7156648189782706No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG389430.6931145248811438No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG364190.6481919184871832No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA322520.5740269023050779No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG316160.5627072598064412No Hit
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG257300.4579471721539642No Hit
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGAGATCGGA221080.39348216408782904No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC215280.38315921967083333No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA195140.34731368509181726No Hit
AGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCA190520.3390909259182793No Hit
ATGAAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACA190230.3385747786974295No Hit
TTTCCGGCAGGCGAAGGTGAAACCTTTCAGCCGCTGACCTGGGAACTGAT185090.32942651416236785No Hit
AAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCG179350.31921035882554794No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT172130.3063600728443912Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAA165030.29372336502358615No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG163900.2917121706802749No Hit
GGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCAC152990.27229435626830545No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA140830.2506517693526731Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA139670.24858718046927392No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG108400.1929322715176437No Hit
TCCGGCAGGCGAAGGTGAAACCTTTCAGCCGCTGACCTGGGAACTGATCA106520.18958621367213474No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC102690.18276951072091174No Hit
ACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCA100610.17906748927481672No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC98720.17570363324927846Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA97950.17433317338702214No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAGATCGGA96120.1710761064416597No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCAGATCGGAAGA94710.16856656305752798No Hit
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGAGATCGGAAGA86280.15356269729282562No Hit
ATGGCGTCGGCAAGACCCGTTCTAAAGCCATCCTGGCTGCAGCGGGTATC85420.15203205381030557No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT85410.15201425563027626No Hit
GGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAG83930.14938012498593944No Hit
AACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGG76450.13606708632402084No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA76120.13547974638305385No Hit
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGTAGATCGG75690.13471442464179384No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG75580.1345186446614715No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG74030.13175992675692955No Hit
TTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAA73980.13167093585678302No Hit
ACAATGAAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGC73220.13031827417455602No Hit
ACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGGA72950.12983772331376484No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGA72740.12946396153314949No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTAGATCGGAAGAGCAC69640.12394652572406556No Hit
AAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAA68770.1223980840615162No Hit
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGAAGATCGGAAG68320.12159716596019758No Hit
AAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGT66850.11898083349589003No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG66540.11842908991498166No Hit
ATTTCCGGCAGGCGAAGGTGAAACCTTTCAGCCGCTGACCTGGGAACTGA65470.11652468465184623No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGA65280.11618651923128948No Hit
AAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAG64710.11517202296961922No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG63260.1125912868653703No Hit
AAAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGTAGATCG60880.10835532001839619No Hit
AGGACGGGGATCAAGAGAGGTCAGATCGGAAGAGCACACGTCTGAACTCC57890.10303366418963461Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATAGATCGG57030.10150302070711457No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)