FastQCFastQC Report
Fri 14 Jul 2023
SRR9691678_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691678_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5220087
Total Bases396.7 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG1562172.9926129583664025No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA1012001.9386650069242142No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA726621.391969137679123No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA552491.0583923218137936No Hit
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC375860.7200263137376829No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA372120.7128616821903543Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG355080.6802185480816698No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG339520.650410615761768No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA297900.5706801438366832No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG285070.5461020094109542No Hit
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG232700.44577801097951053No Hit
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGAGATCGGA200320.3837483934654729No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC192150.36809731332064005No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA180790.34633522391485044No Hit
AGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCA177570.3401667443473643No Hit
ATGAAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACA177270.3395920412820706No Hit
TTTCCGGCAGGCGAAGGTGAAACCTTTCAGCCGCTGACCTGGGAACTGAT168500.3227915550066503No Hit
AAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCG159030.30465009491221123No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT157080.3009145249878019Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAA151080.28942046368192714No Hit
GGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCAC149240.2858956182147922No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG148300.2840948819435385No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA126220.24179673633791926Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA123190.23599223537845251No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG96880.18559077655219156No Hit
TCCGGCAGGCGAAGGTGAAACCTTTCAGCCGCTGACCTGGGAACTGATCA96040.18398160796936908No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC95450.18285135860762475No Hit
ACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCA90390.17315803357300366No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA90010.17243007635696495No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC89090.17066765362339748Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAGATCGGA86190.1651121906588913No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCAGATCGGAAGA84150.1612042098148939No Hit
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGAGATCGGAAGA81180.15551464946848587No Hit
ATGGCGTCGGCAAGACCCGTTCTAAAGCCATCCTGGCTGCAGCGGGTATC79820.15290932890582093No Hit
GGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAG79300.15191317692597844No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT78440.15026569480546972No Hit
AACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGG72470.1388291038061243No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG72390.1386758496553793No Hit
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGTAGATCGG71830.13760307060016433No Hit
TTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAA71660.13727740552983123No Hit
ACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGGA70500.13505522034402875No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG70120.13432726312799No Hit
ACAATGAAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGC67470.12925071938456198No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA67400.1291166220026601No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGA67140.12861854601273887No Hit
AAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAA63930.12246922321409584No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG63370.12139644415888089No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTAGATCGGAAGAGCAC63070.12082174109358715No Hit
AAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGT62810.12032366510366589No Hit
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGAAGATCGGAAG62230.11921257251076467No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG61470.11775665807868718No Hit
AAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAG59570.1141168719984935No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGA58730.11250770341567104No Hit
ATTTCCGGCAGGCGAAGGTGAAACCTTTCAGCCGCTGACCTGGGAACTGA58120.11133914051624043No Hit
AAAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGTAGATCG55870.1070288675265374No Hit
GATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCA54730.1048449958784212No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)