FastQCFastQC Report
Fri 14 Jul 2023
SRR9691679_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691679_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5668106
Total Bases430.7 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG1361732.402442720725406No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA1012041.7854994243226927Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA957331.688976882224856No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA814331.4366880224187761No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA738941.3036806298259067No Hit
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC380180.6707355155319961No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG326290.5756596647980825No Hit
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAA295110.5206501078137917No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG291590.5144399204954883No Hit
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG284850.5025488231871458No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA263850.46549941020863056Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGAGATCGGA261000.4604712755901178No Hit
AAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCG209190.36906508099883806No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAGATCGGA204600.3609671378763912No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA204570.36091421014356473No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA170000.29992381934988516No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG163760.28891485092198343No Hit
AAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAA160410.28300458742302986No Hit
GGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAG157020.27702375361364096No Hit
AGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCA153540.2708841366057727No Hit
GGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCAC147340.2599457384883063No Hit
TGTGGGCACTCGAAGATACGGATTCTTAAGATCGGAAGAGCACACGTCTG141320.24932490676779864Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCA141040.24883091459475176No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG133960.23633996964770948No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG128520.22674240742851315No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA126560.2232844622171851No Hit
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGAGATCGGAAGA122670.21642149952735534No Hit
TTTCCGGCAGGCGAAGGTGAAACCTTTCAGCCGCTGACCTGGGAACTGAT121740.2147807398097354No Hit
ATGAAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACA119270.21042302314035763No Hit
ATGGCGTCGGCAAGACCCGTTCTAAAGCCATCCTGGCTGCAGCGGGTATC113470.2001903281272439No Hit
AATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAG112490.19846135552157987No Hit
AAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAG100680.17762547136556728No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC100560.17741376043426146No Hit
AACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGG100060.17653163155382062No Hit
AGCTCTTGATCCGGCAAACAAACCACCGCTGAGATCGGAAGAGCACACGT97900.1727208347903162No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC84100.14837407769014904Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGGA81910.14451035319381816No Hit
TTAGGACGGGGATCAAGAGAGGTCAGATCGGAAGAGCACACGTCTGAACT79730.14066427127509612Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCTCTTGATCCGGCAAACAAACCACCGCTAGATCGGAAGAGCACACGTC79720.14064662869748729No Hit
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA77510.13674761904593882Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCA76880.13563613665658333No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG74270.13103142390068218No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA73530.12972587315762973No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTAGATCGGAAGAGCAC72440.12780283219826868No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC71050.12535051391064317No Hit
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGAAGATCGGAAG70200.12385089481389375No Hit
AGGACGGGGATCAAGAGAGGTCAGATCGGAAGAGCACACGTCTGAACTCC68490.12083401404278607Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGA68490.12083401404278607No Hit
AAGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGA68230.12037530702495684No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCAGATCGGAAGA66010.11645865479579952No Hit
TAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCG64320.11347705917990948No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG63010.11116588151315449No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA63000.11114823893554568No Hit
TTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAA61120.10783143434508811No Hit
AAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGA59940.10574961018724773No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA58070.10245044817439901Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)