FastQCFastQC Report
Fri 14 Jul 2023
SRR9691681_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691681_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5616295
Total Bases426.8 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG1335682.3782226539026174No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA1017921.8124404077777254Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA933601.6623058439772127No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA807621.4379942649023956No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA731921.3032078977332922No Hit
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC383670.683137192757859No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG323990.5768749682842514No Hit
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAA292530.5208593921793638No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG286420.5099803340102327No Hit
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG281570.5013447477385002No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA264730.4713605677764434Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGAGATCGGA257880.45916391500090364No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAGATCGGA208600.3714192363470936No Hit
AAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCG203720.36273023407780397No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA190410.33903133649496686No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA167900.2989515329946166No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG163030.290280336057846No Hit
AAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAA157900.2811462004755805No Hit
GGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAG155700.2772290273213925No Hit
AGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCA149840.2667951024652373No Hit
GGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCAC148310.2640708865898248No Hit
TGTGGGCACTCGAAGATACGGATTCTTAAGATCGGAAGAGCACACGTCTG142810.2542779537043549Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCA138770.2470845993666643No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG130370.232128120050674No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG129180.23000928548090868No Hit
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGAGATCGGAAGA123550.21998488327269133No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA122590.21827557135086387No Hit
ATGAAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACA118640.21124246500584462No Hit
TTTCCGGCAGGCGAAGGTGAAACCTTTCAGCCGCTGACCTGGGAACTGAT115340.2053667052745627No Hit
AATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAG112130.1996511935359521No Hit
ATGGCGTCGGCAAGACCCGTTCTAAAGCCATCCTGGCTGCAGCGGGTATC110530.1968023403329063No Hit
AACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGG101930.18148975436653522No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC100670.17924628246913668No Hit
AAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAG98360.17513325065723934No Hit
AGCTCTTGATCCGGCAAACAAACCACCGCTGAGATCGGAAGAGCACACGT87410.1556364115488948No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC84110.14976065181761286Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGGA80950.14413416674159743No Hit
TTAGGACGGGGATCAAGAGAGGTCAGATCGGAAGAGCACACGTCTGAACT78550.13986088693702878Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA77500.13799132702252997Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTCTTGATCCGGCAAACAAACCACCGCTAGATCGGAAGAGCACACGTC76120.135534191134903No Hit
GATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCA73250.13042406070193963No Hit
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGAAGATCGGAAG71480.12727251684607024No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA71370.12707665818836084No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG70620.12574125824943314No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTAGATCGGAAGAGCAC69760.12420999965279601No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC69150.1231238743691348No Hit
AGGACGGGGATCAAGAGAGGTCAGATCGGAAGAGCACACGTCTGAACTCC69110.12305265303905867Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGA69070.12298143170898251No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCAGATCGGAAGA66340.11812057593128565No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGA65210.11610857335663458No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA64060.11406096011694541No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG62670.11158601889679941No Hit
TAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCG62400.11110527491878543No Hit
TTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAA60150.10709907510200231No Hit
AAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGA59110.10524732052002254No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA57680.10270115796980038Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)